||Recombinant Human PLATInactive has the active site mutation S478A which renders it catalyticallyinactive, and It is > 90% single chain and retains exosite binding as wellas other properties of wild type PLAT.
||Tissue plasminogenactivator (abbreviated tPA or PLAT) is a protein involved in the breakdown ofblood clots. It is a serine protease found on endothelial cells, the cellsthat line the blood vessels. As an enzyme, it catalyzes the conversion ofplasminogen to plasmin, the major enzyme responsible for clot breakdown.Because it works on the clotting system, PLATis used in clinical medicine to treat only embolic or thrombotic stroke. Useis contraindicated in hemorrhagic stroke and head trauma.
||Liquid in 0.4 MHEPES + 0.1 M NaCl, pH 7.4
||Store in workingaliquots at -80°C. Avoid repeated freeze-thaw cycles.