Recombinant Human Interferon, Alpha 2, HQ-tagged IFNA2-139H

Recombinant Human Interferon, Alpha 2, HQ-tagged


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Recombinant Human Interferon, Alpha 2, HQ-tagged

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Cat. No. : IFNA2-139H
Product Overview : Recombinant human IFNA2molecular weight was 24 KDa.
Description : As a result ofglycosylation, the recombinant protein migrates with an apparent molecular massof 24 kDa in SDS-PAGE. ecombinant human IFNA2a contains 166 amino acids and a16 a.a. HQ tag for a total length of 182 a.a. and has a predicted molecular massof 21.5 kDa including HQ tag.
Source : Produced in theendosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50times less protease activity than E. coli or mammalian cells. Barley seed isvoid of any endotoxin, human or animal viral contaminants.
Species : Human
Form : Sterile filteredthrough a 0.2 μm filter. Lyophilized from PBS, pH 7.2.
Molecular Weight : 24 kDa
Purity : > 95% by SDS-PAGEgel analysis. Purified product carries no pyrogenic or proinflammatory contaminants,as assayed with monocyte activation test using Human 10-plex Cytokine Assaymeasuring IL-6, TNF-alpha and IL-1beta induction.
Endotoxin Level : Endotoxin level isless than 0.005 ng per μg of product (0.05 EU/μg) as measured by turbidimetrickinetic assay. Purified IFNA2a carries no pyrogenic or proinflammatory contaminants,as assayed with monocyte activation test using Human 10-plex Cytokine Assaymeasuring IL-6, TNF-alpha and IL-1beta induction.
Biological Activity : Bioactivity isassayed by measuring the dose dependent cytotoxicity of human IFNA2a on TF-1in the presence of IL-4. The ED50 for this batch of recombinant human IFNA2awas determined to be 0.108 ng/ml, corresponding to specific activity of 9.26x 106 U/mg.
Specific Activity : 9.26 x 106U/mg
Reconstitution : Always centrifugethe vial before opening. It is recommended to reconstitute the lyophilizedprotein in sterile water to a concentration of no less than 100 μg/ml. Forlong term storage it is recommended to add a carrier protein (0.1% HSA orBSA). Please note that the addition of any carrier protein into this productmay produce unwanted endotoxin. This depends upon the particular applicationemployed.
Storage : The lyophilizedprotein, though stable at room temperature for few weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
OfficialSymbol : IFNA2
Gene Name : IFNA2 interferon, alpha 2 [ Homosapiens ]
Synonyms : IFNA2; interferon,alpha 2; IFNA;INFA2; MGC125764; MGC125765; IFN-alphaA; interferon alpha-2; leIF A; IFN-alpha-2;OTTHUMP00000021143; interferon alpha A; alpha-2a interferon; interferon alpha2b
Gene ID : 3440
mRNA Refseq : NM_000605
Protein Refseq : NP_000596
MIM : 147562
UniProt ID : P01563
Chromosome Location : 9p22
Pathway : Autoimmune thyroiddisease; Cytokine Signaling in Immune system; Cytosolic DNA-sensing pathway;Factors involved in megakaryocyte development and platelet production;Hemostasis; Hepatitis C; Herpes simplex infection; Immune System; InfluenzaA; Innate Immune System; Interferon Signaling; Interferon alpha/betasignaling; Jak-STAT signaling pathway; Natural killer cell mediatedcytotoxicity; Regulation of autophagy
Function : cytokine activity;interferon-alpha/beta receptor binding; protein binding

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