| Species : |
Human |
| Source : |
Barley Grain |
| Tag : |
HQ |
| Description : |
As a result ofglycosylation, the recombinant protein migrates with an apparent molecular massof 24 kDa in SDS-PAGE. ecombinant human IFNA2a contains 166 amino acids and a16 a.a. HQ tag for a total length of 182 a.a. and has a predicted molecular massof 21.5 kDa including HQ tag. |
| Form : |
Sterile filteredthrough a 0.2 μm filter. Lyophilized from PBS, pH 7.2. |
| Molecular Weight : |
24 kDa |
| Purity : |
> 95% by SDS-PAGEgel analysis. Purified product carries no pyrogenic or proinflammatory contaminants,as assayed with monocyte activation test using Human 10-plex Cytokine Assaymeasuring IL-6, TNF-alpha and IL-1beta induction. |
| Endotoxin Level : |
Endotoxin level isless than 0.005 ng per μg of product (0.05 EU/μg) as measured by turbidimetrickinetic assay. Purified IFNA2a carries no pyrogenic or proinflammatory contaminants,as assayed with monocyte activation test using Human 10-plex Cytokine Assaymeasuring IL-6, TNF-alpha and IL-1beta induction. |
| Biological Activity : |
Bioactivity isassayed by measuring the dose dependent cytotoxicity of human IFNA2a on TF-1in the presence of IL-4. The ED50 for this batch of recombinant human IFNA2awas determined to be 0.108 ng/ml, corresponding to specific activity of 9.26x 106 U/mg. |
| Specific Activity : |
9.26 x 106U/mg |
| Reconstitution : |
Always centrifugethe vial before opening. It is recommended to reconstitute the lyophilizedprotein in sterile water to a concentration of no less than 100 μg/ml. Forlong term storage it is recommended to add a carrier protein (0.1% HSA orBSA). Please note that the addition of any carrier protein into this productmay produce unwanted endotoxin. This depends upon the particular applicationemployed. |
| Storage : |
The lyophilizedprotein, though stable at room temperature for few weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| OfficialSymbol : |
IFNA2 |
