| Cat. No. : |
IFNA2-139H |
| Description : |
As a result ofglycosylation, the recombinant protein migrates with an apparent molecular massof 24 kDa in SDS-PAGE. ecombinant human IFNA2a contains 166 amino acids and a16 a.a. HQ tag for a total length of 182 a.a. and has a predicted molecular massof 21.5 kDa including HQ tag. |
| Source : |
Produced in theendosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50times less protease activity than E. coli or mammalian cells. Barley seed isvoid of any endotoxin, human or animal viral contaminants. |
| Species : |
Human |
| Form : |
Sterile filteredthrough a 0.2 μm filter. Lyophilized from PBS, pH 7.2. |
| Molecular Weight : |
24 kDa |
| Purity : |
> 95% by SDS-PAGEgel analysis. Purified product carries no pyrogenic or proinflammatory contaminants,as assayed with monocyte activation test using Human 10-plex Cytokine Assaymeasuring IL-6, TNF-alpha and IL-1beta induction. |
| Endotoxin Level : |
Endotoxin level isless than 0.005 ng per μg of product (0.05 EU/μg) as measured by turbidimetrickinetic assay. Purified IFNA2a carries no pyrogenic or proinflammatory contaminants,as assayed with monocyte activation test using Human 10-plex Cytokine Assaymeasuring IL-6, TNF-alpha and IL-1beta induction. |
| Biological Activity : |
Bioactivity isassayed by measuring the dose dependent cytotoxicity of human IFNA2a on TF-1in the presence of IL-4. The ED50 for this batch of recombinant human IFNA2awas determined to be 0.108 ng/ml, corresponding to specific activity of 9.26x 106 U/mg. |
| Specific Activity : |
9.26 x 106U/mg |
| Reconstitution : |
Always centrifugethe vial before opening. It is recommended to reconstitute the lyophilizedprotein in sterile water to a concentration of no less than 100 μg/ml. Forlong term storage it is recommended to add a carrier protein (0.1% HSA orBSA). Please note that the addition of any carrier protein into this productmay produce unwanted endotoxin. This depends upon the particular applicationemployed. |
| Storage : |
The lyophilizedprotein, though stable at room temperature for few weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| OfficialSymbol : |
IFNA2 |
