|Cat. No. :
|Product Overview :
||Recombinant humanJAK2, containing the catalytic and the pseudo kinase domain (aa 532-1132),with N-terminal GST-His Tag.
||JAK2 is a member ofintracellular non-receptor tyrosine kinases that transduce cytokine-mediatedsignals via the JAK-STAT pathway. JAK2 has two near-identicalphosphate-transferring domains. One domain exhibits the kinase activity whilethe other stabilizes the JAK conformational structure. JAK2 is thepredominant JAK kinase activated in response to several growth factors andcytokines such as IL-3, GM-CSF and erythropoietin. JAK2 has been found to beconstitutively associated with the prolactin receptor and is required forresponses to gamma interferon.
||Liquid in 25 mMTris-HCl, pH 8.0 + 100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 3 mM DTT.
|Molecular Weight :
|Specific Activity :
||0.2 U/µg. One unitis defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosinesubstrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4 +3 mM MgCl2 + 3 mM MnCl2 + 1 mM DTT + 3 µM Na-orthovanadate + 0.5 mM ATP + 30µg/ml Poly (Glu:Tyr) 4:1 substrate + 10 µg/ml recombinant JAK2.
||Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.