||Recombinant human DUSP18protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||DUSP18is a member of the dual-specificity phosphatase (DSP) family, which catalyzesdephosphorylation of phosphotyrosine and phosphothreonine residues. DUSP18 isinhibited by iodoarectic acid and is activated by manganese ions.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1mM PMSF, 1mMEDTA.
||23.6 kDa(212aa) confirmed by MALDI-TOF
||> 95%by SDS-PAGE
||0.5mg/ml (determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEVVNTLYEDIQYMQVPVADSPNSRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSGFWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.