"Jag2" Related Products

Expand
Expand

Recombinant Mouse Jagged 2, Fc-tagged

Cat. No.: Jag2-5666M
Product Overview: RecombinantMouse Jag2 protein was expressed in Murine myeloma cell line with theFc part of Mouse IgG2A Glu98-Lys330 (C-terminus) and Mouse Jag2 Met27-Lys1084(N-terminus).
Description: MouseJag2 is a 150 kDa member of the Delta-Serrate-Lag-2 (DSL) family of ligandsthat activate LIN12/Notch proteins, and thereby regulate cell fatedeterminations during development. It is a type 1 transmembrane protein thatis synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signalsequence, a large, 1059 aa extracellular region, a 21 aa transmembraneregion, and a short, 144 aa cytoplasmic region. The extracellular regioncontains four potential N-linked glycosylation sites, a DSL domain, 16EGF-like repeats, many of which are also sites of calcium binding, and acysteine-rich region just proximal to the transmembrane segment.
Source: Murine myeloma cellline, NS0-derived.
Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS and EDTA.
N-terminalSequence: Met27
MolecularWeight: 140.8 kDa (monomer)
Activity: Measuredby the ability of immobilized protein to enhance BMP-2 induced alkalinephosphatase activity in C3H10T1/2 mouse cells. The ED50 for this effect istypically 0.3-1.2 μg/mL. Measured by its ability to bind rrNotch-1 in afunctional ELISA.
Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE: 160-165kDa, reducing conditions.
Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: 100 μg/mLin sterile PBS
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.
Gene Name: Jag2 jagged 2 [ Mus musculus]
Official Symbol: Jag2
Synonyms: Jag2; jagged 2; sm; Serh;D12Ggc2e; mJagged2-1; protein jagged-2; syndactylism
Gene ID: 16450
mRNA Refseq: NM_010588
Protein Refseq: NP_034718
UniProt ID: Q9QYE5
Chromosome Location: 12
Pathway: Delta-NotchSignaling Pathway; Notch Signaling Pathway; Notch receptor binds with aligand; Notch signaling pathway; Receptor-ligand binding initiates the secondproteolytic cleavage of Notch receptor; Signal Transduction; Signaling byNotch
Function: Notch binding;growth factor activity

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.