Recombinant Human Microfibrillar Associated Protein 5, His-tagged

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Recombinant Human Microfibrillar Associated Protein 5, His-tagged

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Cat. No. : MFAP5-5689H
Product Overview : Recombinant Human MFAP5protein was expressed in Murine myeloma cell line with a C-terminal 6-Histag. Ile22-Leu173.
Description : MFAP5 is a secreted,cell-associated 25 kDa mammalian member of the MFAP family of proteins. HumanMFAP5 cDNA encodes 173 amino acids (aa) including a 21 aa signal sequence, anRDG motif (aa 30-32; absent in MAGP1) and a central cysteine-rich binding domain(aa 84-140). Mature human MFAP5 shares 80%, 82%, 82%, 84% and 85% aa identitywith mouse, rat, bovine, porcine and canine MFAP5, respectively.
Source : Murine myeloma cellline, NS0-derived.
Form : Lyophilized from a0.2 μm filtered solution in PBS.
N-terminalSequence : Ile22 & Ser154
Molecular Weight : 18.1 kDa
Activity : Measuredby the ability of the immobilized protein to support the adhesion of BUD-8human skin fibroblast cells. When 2 x 104 cells /well are added toRecombinant Human MFAP5 coated plates (10 μg/ mL, with 100 μL/ well),approximately 50-75% will adhere after one hour at 37°C.
Endotoxin Level : <1.0 EUper 1 μg of the protein by the LAL method.
SDS-PAGE : 25-35kDa, reducing conditions.
Purity : >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : 400μg/mL in PBS
Storage : Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.
Gene Name : MFAP5 microfibrillar associatedprotein 5 [ Homo sapiens ]
Official Symbol : MFAP5
Synonyms : MFAP5; microfibrillarassociated protein 5; MP25; MAGP2; microfibrillar-associated protein 5;MAGP-2; MFAP-5; microfibril-associated glycoprotein 2; microfibril-associatedglycoprotein-2; OTTHUMP00000239218; OTTHUMP00000239219; OTTHUMP00000239220;OTTHUMP00000239266; OTTHUMP00000239268; OTTHUMP00000239269;OTTHUMP00000239270; OTTHUMP00000239271; OTTHUMP00000239272
Gene ID : 8076
mRNA Refseq : NM_003480
Protein Refseq : NP_003471
MIM : 601103
UniProt ID : Q13361
Chromosome Location : 12p13.1-p12.3
Pathway : Notch signaling pathway
Function : extracellular matrix structural constituent

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