Active Recombinant Human Neuroligin 2, His-tagged

• Cat.No.: NLGN2-5692H
• Product Overview: Recombinant Human NLGN2protein was expressed in Murine myeloma cell line with a C-terminal 6-Histag. Gln15-Ser660.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 15-660 a.a.
• Description: NLGN2 is one of severaltype I transmembrane Neuroligins that are expressed on neuronal postsynapticdensities. Neuroligins play an important role in synaptic development andfunction. Mature human NLGN2 is a 105 kDa protein that consists of a 663amino acid extracellular domain with a catalytically inactive cholinesterase-likedomain, a 21 aa transmembrane segment, and a 137 aa cytoplasmic tail. Withinthe ECD, human NLGN2 shares 98% aa sequence identity with mouse and ratNLGN2.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Gln15 predicted
• Molecular Weight: 72 kDa
• Activity: Measuredby its binding ability in a functional ELISA. When recombinant human NLGN2 is immobilized at 1.5 μg/mL, recombinant human Neurexin1β Fc Chimera binds with an apparent KD < 10 nM.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 85-95kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 400μg/mL in PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterile conditionsafter reconstitution.

Gene Information

• Gene Name: NLGN2 neuroligin 2 [ Homosapiens ]
• Official Symbol: NLGN2
• Synonyms: NLGN2; neuroligin 2;KIAA1366; neuroligin-2; OTTHUMP00000135277; EC 3.1.1; EC 3.1.1.1
• Gene ID: 57555
• mRNA Refseq: NM_020795
• Protein Refseq: NP_065846
• MIM: 606479
• UniProt ID: Q8NFZ4
• Chromosome Location: 17p13.2
• Pathway: Cell adhesion molecules (CAMs)
• Function: NOT carboxylesterase activity; cell adhesionmolecule binding; neurexin family protein binding; receptor activity