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Recombinant Mouse Follistatin

Cat. No. : Fst-560M
Product Overview : Recombinantmouse Fst protein was expressed in Spodoptera frugiperda, Sf 21 (baculovirus)-derived,with an N-terminus Met. Gly30-Asn317.
Description : Fst was initiallyidentified as a folliclestimulating hormone inhibiting substance found in ovarianfollicular fluid. It has since been shown that Fst is a high-affinity activin-bindingprotein that can act as an activin antagonist. Two alternatively splicedfollistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS-288)and 315 aa residues (FS-315), exist.
Source : Spodopterafrugiperda, Sf 21 (baculovirus)-derived
Form : Lyophilizedfrom a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
N-terminal Sequence : Met
Molecular Weight : 31 kDa
Activity : Measuredby its ability to neutralize Activin-mediated erythroid differentiation of K562human chronic myelogenous leukemia cells. The ED50 for this effectis typically 0.1-0.4 μg/mL in the presence of 7.5 ng/mL rhActivin A.
Endotoxin Level : <1.0 EU per 1 μgof the protein by the LAL method.
SDS-PAGE : 35-40 kDa, reducingconditions
Purity : >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstitute at 10μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage : Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month,2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions afterreconstitution.
Structureof the follistatin/actinic complex based on PDB 2b0u. :

Gene Name : Fst follistatin [ Musmusculus ]
Official Symbol : Fst
Synonyms : Fst; follistatin;AL033346;FS; activin-binding protein
Gene ID : 14313
mRNA Refseq : NM_008046
Protein Refseq : NP_032072
UniProt ID : P47931
Chromosome Location : 13
Pathway : TGF Beta Signaling Pathway
Function : activin binding; binding; heparin sulfateproteoglycan binding

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