Active Recombinant Mouse Fibroblast Growth Factor 4

• Cat.No.: Fgf4-521M
• Product Overview: RecombinantMouse Fgf4 protein was expressed in E. coli. Ala30-Leu202.

Specification

• Cat. No.: Fgf4-521M
• Description: Fgf4 is a 25kDa secreted, heparin-binding member of the FGF family. The mouse Fgf4 cDNA encodes202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature proteinwith an FGF homology domain that contains a heparin binding region near the C-terminus.Expression of Fgf4 and its receptors, FGF R1c, 2c, 3c and 4,is spatially and temporally regulated during embryonic development. Itsexpression in the trophoblast inner cell mass promotes expression of FGF R2,and is required for maintenance of the trophectoderm and primitive endoderm.
• Source: E. coli
• Form: Lyophilizedfrom a 0.2 um filtered solution in MOPS, Ammonium Sulfate, EDTA, DTT with BSAas a carrier protein.
• N-terminalSequence: Ala30
• MolecularWeight: 19.0 kDa
• Activity: Measuredin a cell proliferation assay using NR6R3T3 mouse fibroblasts. The ED50 forthis effect is typically 0.75-3.75 ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 19 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Fgf4 fibroblast growth factor 4[ Mus musculus ]
• Official Symbol: Fgf4
• Synonyms: Fgf4; fibroblastgrowth factor 4; KS3; hst; Fgfk; Hst1; kFGF; Fgf-4; hst-1; Hstf-1; HBGF-4;K-fibroblast growth factor; heparin-binding growth factor 4
• Gene ID: 14175
• mRNA Refseq: NM_010202
• Protein Refseq: NP_034332
• UniProt ID: P11403
• Chromosome Location: 7
• Pathway: Downstream signalingof activated FGFR; ESC Pluripotency Pathways; FGFR ligand binding andactivation; FGFR1 ligand binding and activation; FGFR1c ligand binding andactivation; FGFR2 ligand binding and activation; FGFR2c ligand binding andactivation; FGFR3 ligand binding and activation; FRS2-mediated cascade; IRS-mediatedsignalling; Insulin receptor signalling cascade; MAPK signaling pathway; Melanoma;PI-3K cascade; Pathways in cancer; Regulation of Actin Cytoskeleton
• Function: fibroblast growthfactor receptor binding; growth factor activity