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Active Recombinant Mouse Granzyme B, His-tagged

Cat.No. : Gzmb-1966M
Product Overview : RecombinantMouse Gzmb protein was expressed in Murine myeloma cell line. Lys17-Ser247,with a C-terminal 10-His tag.
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Cat. No. : Gzmb-1966M
Description : Gzmb is amember of the granzyme family of the serine proteases found specifically inthe cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer(NK) cells. Gzmb plays anessential role in granule-mediated. Apoptosis and may have additional rolesin rheumatoid arthritis and in bacterial and viral infections. It activates variouscaspases and cleaves proteins such as aggrecan. Mouse Gzmb issynthesized as a precursor (247 residues) with a signal and a pro peptide(residues 1-20) and a mature chain (residues 21-247). The recombinant mouse Gzmbconsisting of residues 17 to 247 was expressed and purified. After activationwith cathepsin C, it cleaves a thioester substrate described in the ActivityAssay Protocol.
Source : Mammalian cells
Form : Supplied as a 0.2 μmfiltered solution in HEPES, NaCl and CaCl2.
N-terminalSequence : Lys17 & Gly19
MolecularWeight : 27 kDa
Activity : Measuredby its ability to cleave a peptide substrate, t-ButyloxycaronylAla-Ala-Asp-ThioBenzylester (Boc-AAD-SBzl), in the presence of 5, 5'-Dithio-bis (2-nitrobenzoic acid)(DTNB). The specific activity is > 2,000 pmoles/min/μg as measured underthe described conditions.
Endotoxin Level : <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE : Multiplebands between 34-38 kDa, reducing conditions.
Purity : >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months, -20 to -70°C understerile conditions after opening.
Gene Name : Gzmb granzyme B [ Musmusculus ]
Official Symbol : Gzmb
Synonyms : Gzmb; granzyme B; GZB;CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11; granzyme B (G, H);fragmentin-2; cytotoxic cell protease 1; EC 3.4.21.79
Gene ID : 14939
mRNA Refseq : NM_013542
Protein Refseq : NP_038570
UniProt ID : P04187
Chromosome Location : 14
Pathway : Activation; Allograftrejection; Apoptosis; Autoimmune thyroid disease; Graft-versus-host disease; IntrinsicPathway for Apoptosis; Natural killer cell mediated cytotoxicity; Oxidativephosphorylation; Transcriptional misregulation in cancer; Type I diabetesmellitus
Function : catalytic activity; hydrolaseactivity; peptidase activity; protein binding; serine-type endopeptidaseactivity; serine-type peptidase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are some potential future applications of GZMB in medicine? 02/05/2020

GZMB may be used in the development of targeted therapies for viral infections and various immune-related disorders.

How is GZMB protein clinically relevant in cancer treatment? 07/28/2018

GZMB can be used in cancer immunotherapy to target and eliminate cancer cells.

What are the challenges in using GZMB in cancer immunotherapy? 09/14/2016

One challenge is to ensure that GZMB selectively targets cancer cells without causing harm to healthy cells.

What types of cancer can be targeted using GZMB-based therapies? 07/24/2016

GZMB-based therapies can be used against various types of cancer, including leukemia, lymphoma, and solid tumors.

Can GZMB be used in autoimmune disease treatment? 03/22/2016

GZMB's role in autoimmune diseases is complex. It can contribute to tissue damage in some autoimmune conditions, but its use in treatment is not common.

Customer Reviews (3)

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Reviews
11/01/2020

    I am delighted to express my complete satisfaction with the quality of GZMB protein, as it precisely suits my experimental requirements.

    08/29/2017

      Their knowledgeable team has been readily available to address any concerns or queries, providing effective solutions and guidance whenever needed.

      07/28/2017

        I have full confidence in the GZMB protein and the exceptional support provided by the manufacturer, making it my top recommendation for researchers seeking a high-quality protein for their experimental needs.

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