||Recombinant human HSD17B8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||HSD17B8, also known as estradiol 17-beta-dehydrogenase 8, belongs to the short-chain dehydrogenases/reductases (SDR) family. In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. It may play a role in biosynthesis of fatty acids in mitochondria.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 150mM NaCl
||29.1 kDa (281aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MASQLQNRLR SALALVTGAG SGIGRAVSVR LAGEGATVAACDLDRAAAQE TVRLLGGPGS KEGPPRGNHAAFQADVSEAR AARCLLEQVQ ACFSRPPSVV VSCAGITQDE FLLHMSEDDW DKVIAVNLKG TFLVTQAAAQ ALVSNGCRGS IINISSIVGKVGNVGQTNYA ASKAGVIGLT QTAARELGRH GIRCNSVLPG FIATPMTQKV PQKVVDKITE MIPMGHLGDP EDVADVVAFL ASEDSGYITGTSVEVTGGLF M
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.