Active Recombinant Mouse Iduronate 2-Sulfatase, His-tagged

• Cat.No.: Ids-1974M
• Product Overview: RecombinantMouse Ids protein was expressed in Murine myeloma cell line. Thr36-Pro552(Asp177-Lys179 deletion), with an N-terminal signal sequence and a C-terminal10-His tag.

Specification

• Cat. No.: Ids-1974M
• Description: As amember of the sulfatase family, Iduronate 2-Sulfatase encoded by the Ids gene isrequired for the lysosomal degradation of the glycosaminoglycans (GAG)heparan sulfate and dermatan sulfat. It hydrolyzes the 2-sulfate group of theL-iduronate 2-sulfate units of the GAG. The Idsdeficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome),an X-linked inborn error leading to lysosomal accumulation of the GAG andtheir excretion in urine. MPS II has a wide spectrum of clinicalmanifestations ranging from mild to severe. The mouse Ids hassulfatase activity described in the Activity Assay Protocol.
• Source: Mammalian cells
• Form: Supplied as a 0.2 umfiltered solution in Tris and NaCl.
• N-terminalSequence: Thr36
• MolecularWeight: 60 kDa
• Activity: Measuredby its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). Thespecific activity is > 1.0 pmol/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 77 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freezethawcycles. 6months from date of receipt, -20 to -70°C as supplied. 3 months, -20 to -70°Cunder sterile conditions after opening.

Gene Information

• Gene Name: Ids iduronate 2-sulfatase [ Musmusculus ]
• Official Symbol: Ids
• Synonyms: Ids;iduronate 2-sulfatase; AW214631; iduronate sulfatase;iduronate-2-sulfatase; alpha-L-iduronate sulfate sulfatase; EC 3.1.6.13
• Gene ID: 15931
• mRNA Refseq: NM_010498
• Protein Refseq: NP_034628
• UniProt ID: Q08890
• Chromosome Location: X
• Pathway: Dermatan sulfatedegradation; Glycosaminoglycan degradation; Heparan sulfate degradation; Lysosome; Metabolic pathways
• Function: catalytic activity;hydrolase activity; iduronate-2-sulfatase activity; metal ion binding; protein binding; sulfuric ester hydrolaseactivity