"MMEL1" Related Products

Recombinant Human Membrane Metallo-Endopeptidase-Like 1, His-tagged

Cat. No.: MMEL1-1981H
Product Overview: RecombinantHuman MMEL1 protein was expressed in Murine myeloma cellline. Gly69-Trp770,with an N-terminal 6-His tag.
Description: MMEL1 is azinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2,PEX, DINE, Kell and several NEPlike proteins. It is encoded by the MMEL1 genein the human genome and is also known as soluble secreted endopeptidase (SEP),Neprilysinlike 1 and Neprilysin-2. Highly expressed in testis, the cDNApredicted a type II transmembrane protein with a short cytoplasmic tail and alarge ectodomain. Both membranebound and soluble forms were comparable withregard to model substrates, pH optima and inhibitor profiles. The ectodomainof human MMEL1 was expressedwith an N-terminal His tag and purified.
Source: Murine myeloma cellline, NS0-derived.
Form: Supplied as a 0.2 μmfiltered solution in Tris, NaCl and ZnCl2.
N-terminalSequence: His
MolecularWeight: 82 kDa
Activity: Measuredby its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH.The specific activity is > 250 pmoles/min/μg, as measured under thedescribed conditions.
Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE: 99 kDa,reducing conditions.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.
Gene Name: MMEL1 membranemetallo-endopeptidase-like 1 [ Homo sapiens ]
Official Symbol: MMEL1
Synonyms: MMEL1; membranemetallo-endopeptidase-like 1; NL1; NL2; SEP; NEP2; MMEL2; NEPII; NEP2 (m);neprilysin-2; neprilysin II; zinc metallopeptidase; soluble secretedendopeptidase; EC 3.4.24.11
Gene ID: 79258
mRNA Refseq: NM_033467
Protein Refseq: NP_258428
UniProt ID: Q495T6
Chromosome Location: 1p36
Function: metal ion binding; metalloendopeptidaseactivity; peptidase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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