Active Recombinant Human Insulin-Like 3 (Leydig cell)

• Cat.No.: INSL3-384H
• Product Overview: RecombinantHuman INSL3 protein was expressed in E. coli. Ala106-Tyr131(A chain) & Leu21-Ala55.

Specification

• Cat. No.: INSL3-384H
• Description: INSL3 isa member of the insulin/relaxin superfamily. INSL3 is a secreted proteinproduced in high amounts by testicular Leydig cells, and in lower amounts by ovarianfollicular theca interna cells. As with other insulin/relaxin superfamilymembers, human INSL3 is synthesized as a preprohormone. The Prepro INSL3precursor is 131 amino acids (aa) in length. It is processed by a prohormoneconvertase to remove a 20 aa signal sequence and a 47 aa internal pro-, or C-peptidethat separates a 35 aa N-terminal B chain from a 26 aa C-terminal A chain.
• Source: E. coli
• Form: Lyophilizedfrom a 0.2 um filtered solution in PBS.
• N-terminalSequence: Ala106 (A chain)& Leu21(B chain)
• MolecularWeight: 2.8 kDa (A chain),3.3 kDa (B chain)
• Activity: Measuredby its ability to induce cAMP accumulation in human RXFP2 transfected HEK293human embryonic kidney cells. The ED50 for this effect is typically 12.5-50 pg/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: INSL3 insulin-like 3 (Leydigcell) [ Homo sapiens ]
• Official Symbol: INSL3
• Synonyms: INSL3; insulin-like3 (Leydig cell); RLF; RLNL; insulin-like 3; ley-I-L; relaxin-likefactor b; leydig insulin-like peptide; leydig insulin -like hormone
• Gene ID: 3640
• mRNA Refseq: NM_005543
• Protein Refseq: NP_005534
• MIM: 146738
• UniProt ID: P51460
• Chromosome Location: 19p13.2-p12
• Pathway: Class A/1 (Rhodopsin-like receptors);Cytokine Signaling in Immune system; G alpha (s) signalling events; GPCRdownstream signaling; GPCR ligand binding; Immune System; Interleukinreceptor SHC signaling; Interleukin-2 signaling; Interleukin-3, 5 and GM-CSFsignaling; Interleukin-7 signaling; Peptide ligand-binding receptors; Relaxinreceptors; Signal Transduction; Signaling by GPCR; Signaling by Interleukins
• Function: G-protein coupled receptor binding; hormoneactivity; insulin receptor binding; receptor binding