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Active Recombinant Mouse Glypican 5, His-tagged

Cat.No. : Gpc5-5628M
Product Overview : RecombinantMouse Gpc5 protein was expressed in Murine myeloma cell line. Gly26-Gly549,with a C-terminal 6-His tag.
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Cat. No. : Gpc5-5628M
Description : Theglypicans (glypiated proteoglycans) are a small multigene family ofGPI-linked proteoglycans that likely play a key role in embryonicmorphogenesis. There are six known mammalian glypicans. They all share a common-sizedprotein core of 60-70 kDa, an N-terminus which likely forms a compact globulardomain, 14 conserved cysteines that form multiple intrachain disulfide bonds,and a number of C-terminal N-and O-linked carbohydrate attachment sites.Based on exon organization and the location of O-linked glycosylation sites,at least two subfamilies of glypicans are known, with one subfamily containingglypicans-1, 2, 4, and 6, and another subfamily containing glypicans-3 and 5.
Source : Mammalian cells
Form : Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein.
N-terminalSequence : Gly26
MolecularWeight : 59.1 kDa
Activity : Measuredby its binding ability in a functional ELISA. Immobilized rm Gpc5 at5μg/mL (100μL/well) can bind rhFGF-basic with a linear range of 0.16-10ng/mL.
Endotoxin Level : <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE : 61-65kDa, reducing conditions.
Purity : >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstituteat 100μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.
Storage : Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
Gene Name : Gpc5 glypican 5 [ Musmusculus ]
Official Symbol : Gpc5
Synonyms : Gpc5; glypican 5; A230034F01Rik;glypican-5
Gene ID : 103978
mRNA Refseq : NM_175500
Protein Refseq : NP_780709
UniProt ID : O70206
Chromosome Location : 14
Function : heparan sulfateproteoglycan binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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