| Cat. No. : |
Dkk1-395M |
| Description : |
Dkk1 is the foundingmember of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, anda related protein, Soggy. Dkk proteins are secreted proteins that contain twoconserved cysteine-rich domains separated by a linker region. Each domaincontains ten cysteine residues. Mature mouse Dkk1 is a 40 kDa glycosylatedprotein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identitywith human, rat, rabbit and bovine Dkk1, respectively. |
| Source : |
Mammalian cells |
| Form : |
Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| N-terminal Sequence : |
Ser30 |
| Molecular Weight : |
26.1 kDa |
| Activity : |
Measured by itsability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3E1 mouseosteoblastic cells. The ND50 for this effect is typically 0.2-1 μg/mLin the presence of 10 ng/mL of rmWnt-3a. Measured by its ability to interferewith the axisinducing activity of Xwnt-8 mRNA in early Xenopusembryos.In a population of embryos, the addition of 1 ng of rmDkk1 per embryoresulted in 60-100% reduction in the number of secondary axes produced by 10-20pg of Xwnt-8 mRNA. Optimal concentrations should be determined by eachlaboratory for each application. |
| Endotoxin Level : |
<1.0 EU per 1 μgof the protein by the LAL method. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstitute at 100μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin. |
| Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution. |
