"Dkk1" Related Products


Recombinant Mouse Dickkopf Homolog 1 (Xenopus laevis), His-tagged

Cat. No.: Dkk1-395M
Product Overview: Recombinantmouse Dkk1 protein was expressed in Murine myeloma cell line, NS0-derived, with a C-terminal10-His tag. Ser30-His272.
Description: Dkk1 is the foundingmember of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, anda related protein, Soggy. Dkk proteins are secreted proteins that contain twoconserved cysteine-rich domains separated by a linker region. Each domaincontains ten cysteine residues. Mature mouse Dkk1 is a 40 kDa glycosylatedprotein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identitywith human, rat, rabbit and bovine Dkk1, respectively.
Source: Murine myeloma cellline, NS0-derived
Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
N-terminal Sequence: Ser30
Molecular Weight: 26.1 kDa
Activity: Measured by itsability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3E1 mouseosteoblastic cells. The ND50 for this effect is typically 0.2-1 μg/mLin the presence of 10 ng/mL of rmWnt-3a. Measured by its ability to interferewith the axisinducing activity of Xwnt-8 mRNA in early Xenopusembryos.In a population of embryos, the addition of 1 ng of rmDkk1 per embryoresulted in 60-100% reduction in the number of secondary axes produced by 10-20pg of Xwnt-8 mRNA. Optimal concentrations should be determined by eachlaboratory for each application.
Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 100μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.
Gene Name: Dkk1dickkopf homolog 1 (Xenopus laevis) [ Mus musculus ]
Official Symbol: Dkk1
Synonyms: Dkk1; dickkopfhomolog 1; mddk-1; OTTMUSP00000022079; dickkopf-related protein 1; dkk-1; dickkopf-1;mCG_6758
Gene ID: 13380
mRNA Refseq: NM_010051
Protein Refseq: NP_034181
UniProt ID: Q80UL5
Chromosome Location: 19
Pathway: Wnt SignalingPathway; PluriNetWork
Function: Low-density lipoprotein particle binding; receptorantagonist activity; protein binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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