"Dkk1" Related Products

Expand
Expand

Recombinant Mouse Dickkopf Homolog 1 (Xenopus laevis), His-tagged

Cat. No.: Dkk1-395M
Product Overview: Recombinantmouse Dkk1 protein was expressed in Murine myeloma cell line, NS0-derived, with a C-terminal10-His tag. Ser30-His272.
Description: Dkk1 is the foundingmember of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, anda related protein, Soggy. Dkk proteins are secreted proteins that contain twoconserved cysteine-rich domains separated by a linker region. Each domaincontains ten cysteine residues. Mature mouse Dkk1 is a 40 kDa glycosylatedprotein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identitywith human, rat, rabbit and bovine Dkk1, respectively.
Source: Murine myeloma cellline, NS0-derived
Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
N-terminal Sequence: Ser30
Molecular Weight: 26.1 kDa
Activity: Measured by itsability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3E1 mouseosteoblastic cells. The ND50 for this effect is typically 0.2-1 μg/mLin the presence of 10 ng/mL of rmWnt-3a. Measured by its ability to interferewith the axisinducing activity of Xwnt-8 mRNA in early Xenopusembryos.In a population of embryos, the addition of 1 ng of rmDkk1 per embryoresulted in 60-100% reduction in the number of secondary axes produced by 10-20pg of Xwnt-8 mRNA. Optimal concentrations should be determined by eachlaboratory for each application.
Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 100μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.
Gene Name: Dkk1dickkopf homolog 1 (Xenopus laevis) [ Mus musculus ]
Official Symbol: Dkk1
Synonyms: Dkk1; dickkopfhomolog 1; mddk-1; OTTMUSP00000022079; dickkopf-related protein 1; dkk-1; dickkopf-1;mCG_6758
Gene ID: 13380
mRNA Refseq: NM_010051
Protein Refseq: NP_034181
UniProt ID: Q80UL5
Chromosome Location: 19
Pathway: Wnt SignalingPathway; PluriNetWork
Function: Low-density lipoprotein particle binding; receptorantagonist activity; protein binding

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.