Active Recombinant Mouse Prokineticin 1

• Cat.No.: Prok1-396M
• Product Overview: Recombinant mouse Prok1protein was expressed in Murine myeloma cell line, NS0-derived. Ala20-Phe105.

Specification

• Cat. No.: Prok1-396M
• Description: Prok1 is a member ofthe prokineticin family of secreted proteins whose distinguishing structuralmotif involves 10 cysteines that form five intrachain disulfide bonds,creating a "colipase fold". The mature region in mouse is 93% and 87% aaidentical to the mature regions in rat and human, respectively.
• Source: Mammalian cells
• Form: Supplied as a 0.2 μmfiltered solution in NaH2PO4 and NaCl with BSA as acarrier protein.
• N-terminal Sequence: Ala20
• Molecular Weight: 9.6 kDa
• Activity: Measured in a cellproliferation assay using EJG bovine endothelial cells (derived from adrenaltissue). The ED50 for this effect is typically 1-4 μg/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Prok1prokineticin 1 [ Mus musculus ]
• Official Symbol: Prok1
• Synonyms: Prok1; prokineticin 1; EG-VEGF; prokineticin-1;endocrine-gland-derived vascular endothelial growth factor; Pk1
• Gene ID: 246691
• mRNA Refseq: NM_001044382
• Protein Refseq: NP_001037847
• UniProt ID: O14A28
• Chromosome Location: 3
• Pathway: G alpha (q)signaling; GPCR downstream signaling; Signal Transduction; Signaling by GPCR
• Function: Growth factoractivity; G-protein coupled receptor binding