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Recombinant Rat Chemokine (C-C motif) Ligand 20

Cat. No.: Ccl20-409R
Product Overview: Recombinantrat Ccl20 protein was expressed in E. coli. Ala26-Met96.
Description: Ccl20, also known asMIP3α, LARC (Liver and Activationregulated Chemokine) and as Exodus, is oneof many novel β chemokines identified through bioinformatics. Rat Ccl20 cDNAencodes a 96 amino acid (aa) precursor protein with a 25 aa putative signalpeptide that is predicted to be cleaved to form the 71 aa mature secretedprotein.
Source: E. coli
Form: Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
N-terminal Sequence: Ala26
Molecular Weight: 8.2 kDa
Activity: Measured by itsability to chemoattract human CCR6 transfected BaF3 mouse pro-B cells. The ED50for this effect is typically 0.5-3 μg/mL.
Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 200μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month,2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions afterreconstitution.
Gene Name: Ccl20 chemokine (C-C motif)ligand 20 [ Rattus norvegicus ]
Official Symbol: Ccl20
Synonyms: Ccl20; chemokine(C-C motif) ligand 20; ST38; Scya20; MIP-3-alpha; CCchemokine LARC; CC chemokine ST38; beta chemokine exodus-1; small-induciblecytokine A20; macrophage inflammatory protein 3 alpha; liver andactivation-regulated chemokine; beta-chemokine exodus-1; small induciblecytokine subfamily A20; C-C motif chemokine 20
Gene ID: 29538
mRNA Refseq: NM_019233
Protein Refseq: NP_062106
UniProt ID: P97884
Chromosome Location: 9q34
Pathway: Chemokine receptors bind chemokines; Chemokinesignaling pathway; Class A/1 (Rhodopsin-like receptors); Cytokine-cytokinereceptor interaction; G alpha (i) signalling events; GPCR downstreamsignaling; GPCR ligand binding; Peptide ligand-binding receptors; Rheumatoidarthritis; Signal Transduction; Signaling by GPCR
Function: chemokine activity; cytokine activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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