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Recombinant Rat Chemokine (C-C motif) Ligand 20

Cat. No. : Ccl20-409R
Product Overview : Recombinantrat Ccl20 protein was expressed in E. coli. Ala26-Met96.
Description : Ccl20, also known asMIP3α, LARC (Liver and Activationregulated Chemokine) and as Exodus, is oneof many novel β chemokines identified through bioinformatics. Rat Ccl20 cDNAencodes a 96 amino acid (aa) precursor protein with a 25 aa putative signalpeptide that is predicted to be cleaved to form the 71 aa mature secretedprotein.
Source : E. coli
Form : Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
N-terminal Sequence : Ala26
Molecular Weight : 8.2 kDa
Activity : Measured by itsability to chemoattract human CCR6 transfected BaF3 mouse pro-B cells. The ED50for this effect is typically 0.5-3 μg/mL.
Endotoxin Level : <1.0 EU per 1 μgof the protein by the LAL method.
Purity : >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstitute at 200μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage : Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month,2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions afterreconstitution.
PDBrendering based on 1m8a. :

Gene Name : Ccl20 chemokine (C-C motif)ligand 20 [ Rattus norvegicus ]
Official Symbol : Ccl20
Synonyms : Ccl20; chemokine(C-C motif) ligand 20; ST38; Scya20; MIP-3-alpha; CCchemokine LARC; CC chemokine ST38; beta chemokine exodus-1; small-induciblecytokine A20; macrophage inflammatory protein 3 alpha; liver andactivation-regulated chemokine; beta-chemokine exodus-1; small induciblecytokine subfamily A20; C-C motif chemokine 20
Gene ID : 29538
mRNA Refseq : NM_019233
Protein Refseq : NP_062106
UniProt ID : P97884
Chromosome Location : 9q34
Pathway : Chemokine receptors bind chemokines; Chemokinesignaling pathway; Class A/1 (Rhodopsin-like receptors); Cytokine-cytokinereceptor interaction; G alpha (i) signalling events; GPCR downstreamsignaling; GPCR ligand binding; Peptide ligand-binding receptors; Rheumatoidarthritis; Signal Transduction; Signaling by GPCR
Function : chemokine activity; cytokine activity

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