Active Recombinant Mouse Chemokine (C-C motif) Ligand 21A (serine)

• Cat.No.: Ccl21a-412M
• Product Overview: Recombinantmouse Ccl21a protein was expressed in E. coli. Ser24-Gly133.

Specification

• Cat. No.: Ccl21a-412M
• Description: Mouse Ccl21a cDNAencodes a 133 amino acid residue protein with a 23 residue signal peptide thatis cleaved to generate the 110 residue mature protein. Human and mouse Ccl21aare highly conserved, exhibiting 86% amino acid sequence identity. Ccl21a isconstitutively expressed at high levels in lymphoid tissues such as lymphnodes, spleen and appendix.
• Source: E. coli
• Form: Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
• N-terminal Sequence: Ser24
• Molecular Weight: 12 kDa
• Activity: Measured by itsability to chemoattract 5-10 day cultured human peripheral blood lymphocytes(PBL). The ED50 for this effect is typically 50-200 ng/mL. Measuredby its ability to chemoattract human CCR7 transfected BaF3 mouse pro-B cells.The ED50 for this effect is typically 3-15 ng/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 10μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month,2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions afterreconstitution.

Gene Information

• Gene Name: Ccl21a chemokine (C-C motif)ligand 21A (serine) [ Mus musculus ]
• Official Symbol: Ccl21a
• Synonyms: Ccl21a;chemokine (C-C motif) ligand 21A (serine); ALP; SLC; plt; CKb9;Tca4; 6Ckine; Scya21; 6CKBAC2; SCYA21a; Scya21b; AW987545; OTTMUSP00000006949;Exodus-2; C-C motif chemokine 21a;CCL21-Ser; CC chemokine 6Ckine-ser; beta chemokineexodus-2; beta-chemokine exodus-2; smallinducible cytokine A21a; small-inducible cytokine A21a; thymus-derivedchemotactic agent 4; TCA4
• Gene ID: 18829
• mRNA Refseq: NM_011124
• Protein Refseq: NP_035254
• UniProt ID: P844444
• Chromosome Location: 4
• Pathway: Chemokine receptorsbind chemokines; Chemokine signaling pathway; Class A/1 (Rhodopsin-likereceptors); Cytokine-cytokine receptor interaction; G alpha (i) signallingevents; GPCR downstream signaling; GPCR ligand binding; Peptideligand-binding receptors; Signal Transduction; Signaling by GPCR