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Recombinant Rat Pro-Platelet Basic Protein (chemokine (C-X-C motif) ligand 7)

Cat. No.: Ppbp-448R
Product Overview: Recombinant Rat Ppbpprotein was expressed in E. coli. Ile46-Ile107.
Description: Ppbp is amember of the alpha (CXC) subfamily of chemokines. Rat Ppbp cDNAencodes a 111 amino acid residue precursor protein. Rat Ppbp shares72% amino acid sequence identity with mouse Ppbp. The recombinantrat Ppbp producedby R&D Systems has been shown to chemoattract human CXCR2 transfectedmouse BaF/3 cells, but not human CXCR1 transfected mouse BaF/3 cells.
Source: E. coli
Form: Lyophilized from a0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
N-terminalSequence: Ile46
MolecularWeight: 6.8 kDa
Activity: Measuredby its ability to chemoattract human CXCR2 transfected BaF3 mouse proB cells.The ED50 for this effect is typically 1 5 ng/mL.
Endotoxin Level: <1.0EU per 1 μg of the protein by the LAL method.
Purity: >97%,by SDSPAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 25μg/mL in sterile PBS containing at least 0.1%human or bovine serum albumin.
Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, 20 to 70°C as supplied. 1 month,2 to 8°C under sterile conditions after reconstitution. 3 months, 20 to 70°Cunder sterile conditions after reconstitution.
Gene Name: Ppbp pro-platelet basicprotein (chemokine (C-X-C motif) ligand 7) [ Rattus norvegicus ]
Official Symbol: Ppbp
Synonyms: Ppbp; pro-plateletbasic protein (chemokine (C-X-C motif) ligand 7); Cxcl7; Nap-2; plateletbasic protein; neutrophil activating peptide-2; chemokine (C-X-C motif)ligand 7
Gene ID: 246358
mRNA Refseq: NM_153721
Protein Refseq: NP_714943
UniProt ID: Q99ME0
Chromosome Location: 14
Pathway: Chemokine receptorsbind chemokines; Chemokine signaling pathway; Class A/1 (Rhodopsin-likereceptors); Cytokine-cytokine receptor interaction; G alpha (i) signallingevents; GPCR downstream signaling; GPCR ligand binding; Hemostasis; Peptideligand-binding receptors; Platelet activation; Platelet degranulation; SignalTransduction; Signaling by GPCR
Function: chemokine activity;molecular_function

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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