Active Recombinant Rat Pro-Platelet Basic Protein (chemokine (C-X-C motif) ligand 7)

• Cat.No.: Ppbp-448R
• Product Overview: Recombinant Rat Ppbpprotein was expressed in E. coli. Ile46-Ile107.

Specification

• Cat. No.: Ppbp-448R
• Description: Ppbp is amember of the alpha (CXC) subfamily of chemokines. Rat Ppbp cDNAencodes a 111 amino acid residue precursor protein. Rat Ppbp shares72% amino acid sequence identity with mouse Ppbp. The recombinantrat Ppbp producedby R&D Systems has been shown to chemoattract human CXCR2 transfectedmouse BaF/3 cells, but not human CXCR1 transfected mouse BaF/3 cells.
• Source: E. coli
• Form: Lyophilized from a0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
• N-terminalSequence: Ile46
• MolecularWeight: 6.8 kDa
• Activity: Measuredby its ability to chemoattract human CXCR2 transfected BaF3 mouse proB cells.The ED50 for this effect is typically 1 5 ng/mL.
• Endotoxin Level: <1.0EU per 1 μg of the protein by the LAL method.
• Purity: >97%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 25μg/mL in sterile PBS containing at least 0.1%human or bovine serum albumin.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, 20 to 70°C as supplied. 1 month,2 to 8°C under sterile conditions after reconstitution. 3 months, 20 to 70°Cunder sterile conditions after reconstitution.

Gene Information

• Gene Name: Ppbp pro-platelet basicprotein (chemokine (C-X-C motif) ligand 7) [ Rattus norvegicus ]
• Official Symbol: Ppbp
• Synonyms: Ppbp; pro-plateletbasic protein (chemokine (C-X-C motif) ligand 7); Cxcl7; Nap-2; plateletbasic protein; neutrophil activating peptide-2; chemokine (C-X-C motif)ligand 7
• Gene ID: 246358
• mRNA Refseq: NM_153721
• Protein Refseq: NP_714943
• UniProt ID: Q99ME0
• Chromosome Location: 14
• Pathway: Chemokine receptorsbind chemokines; Chemokine signaling pathway; Class A/1 (Rhodopsin-likereceptors); Cytokine-cytokine receptor interaction; G alpha (i) signallingevents; GPCR downstream signaling; GPCR ligand binding; Hemostasis; Peptideligand-binding receptors; Platelet activation; Platelet degranulation; SignalTransduction; Signaling by GPCR
• Function: chemokine activity;molecular_function