Active Recombinant Mouse Erythropoietin Receptor

• Cat.No.: Epor-464M
• Product Overview: Recombinant Mouse Eporprotein was expressed in Murine myeloma cell line. Ala27-Arg192.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Non
• Protein Length: 27-192 a.a.
• Description: Erythropoietin (Epo)is a 34 kDa glycoprotein hormone in the type I cytokine family and is relatedto thrombopoietin. Its three N glycosylation sites, four alpha helices, and NtoCterminal disulfide bond are conserved across species. Glycosylation of Epo isrequired for biological activities in vivo. Mature mouse Epo shares 95% aminoacid sequence identity with rat Epo and 73%-82% with bovine, canine, equine, feline,human, ovine, and porcine EPO.
• Form: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Ala27
• Molecular Weight: 18.6 kDa
• Activity: Measuredin a cell proliferation assay using TF1 human erythroleukemic cells. Kitamura,T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effectis typically 0.4-2 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 10 μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Epor erythropoietinreceptor [ Mus musculus ]
• Official Symbol: Epor
• Synonyms: Epor; erythropoietinreceptor; EPO-R
• Gene ID: 13857
• mRNA Refseq: NM_010149
• Protein Refseq: NP_034279
• UniProt ID: P14753
• Chromosome Location: 9 A3; 9 5.0 cM
• Pathway: Cytokine-cytokine receptorinteraction; EPO Receptor Signaling; Hematopoietic cell lineage; Jak-STAT signalingpathway; Kit Receptor Signaling Pathway
• Function: cytokine receptor activity;erythropoietin receptor activity; identical protein binding; protein binding;receptor activity; transmembrane signaling receptor activity