"LRFN3" Related Products

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Recombinant Human Leucine Rich Repeat And Fibronectin Type III Domain Containing 3, His-tagged

Cat. No.: LRFN3-486H
Product Overview: Recombinanthuman LRFN3 protein with a C-terminal 6-His tag wasexpressed in Murine myeloma cell line, NS0-derived. Ser17 - Met539.
Description: LRFN3 is anapproximately 90 kDa member of the Lrfn family of type I transmembraneglycoproteins. Human LRFN3 is synthesized as a 628 amino acid (aa) precursorthat contains a 16 aa signal sequence, a 523 aa extracellular domain (ECD), a21 aa transmembrane region, and a 68 aa cytoplasmic region. The ECD consistsof six leucinerich repeats (LRR), an IgC2like domain, and a fibronectintypeIII domain, tandemly aligned in that order.
Source: Murine myeloma cellline, NS0-derived
Form: Lyophilized from a0.2 μm filtered solution in PBS.
N-terminalSequence: Ser17
MolecularWeight: 56.3 kDa
Activity: Measured by theability of the immobilized protein to support the adhesion of Neuro2a mouseneuroblastoma cells (ATCC: CCL131). When 5 x 104 cells/well are added torhSALM4 coated plates (2.5 μg/mL, 100 μL/well), approximately 50% 70% willadhere after 30 minutes at 37 °C. Optimal dilutions should be determined byeach laboratory for each application.
Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
Purity: >90%, by SDSPAGE underreducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 200μg/mL in PBS.
Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.
Gene Name: LRFN3 leucine rich repeat andfibronectin type III domain containing 3 [ Homo sapiens ]
Official Symbol: LRFN3
Synonyms: LRFN3; leucinerich repeat and fibronectin type III domain containing 3; SALM4; FIGLER1; leucine-richrepeat and fibronectin type-III domain-containing protein 3; synapticadhesion-like molecule 4; fibronectin type III, immunoglobulin and leucinerich repeat domains 1
Gene ID: 79414
mRNA Refseq: NM_024509
Protein Refseq: NP_078785
MIM: 612809
UniProt ID: Q9BTN0
Chromosome Location: 19q13.13

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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