||Recombinant humanCCNA2 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
||CCNA2 belongs to thehighly conserved cyclin family, whose members are characterized by a dramaticperiodicity in protein abundance through the cell cycle. Cyclins function asregulators of CDK kinases. Different cyclins exhibit distinct expression anddegradation patterns which contribute to the temporal coordination of eachmitotic event. In contrast to cyclin A1, which is present only in germ cells,this cyclin is expressed in all tissues tested. This cyclin binds andactivates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S andG2/M transitions.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol 0.15M NaCl, 1mM DTT
||51.1 kDa (456aa)
||> 90% by SDS -PAGE
||0.5 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMLGNSA PGPATREAGS ALLALQQTAL QEDQENINPE KAAPVQQPRT RAALAVLKSGNPRGLAQQQRPKTRRVAPLK DLPVNDEHVT VPPWKANSKQ PAFTIHVDEA EKEAQKKPAE SQKIEREDALAFNSAISLPG PRKPLVPLDY PMDGSFESPHTMDMSIVLED EKPVSVNEVP DYHEDIHTYL REMEVKCKPKVGYMKKQPDI TNSMRAILVD WLVEVGEEYK LQNETLHLAV NYIDRFLSSMSVLRGKLQLV GTAAMLLASKFEEIYPPEVA EFVYITDDTY TKKQVLRMEH LVLKVLTFDL AAPTVNQFLT QYFLHQQPANCKVESLAMFLGELSLIDADP YLKYLPSVIA GAAFHLALYT VTGQSWPESL IRKTGYTLES LKPCLMDLHQTYLKAPQHAQ QSIREKYKNS KYHGVSLLNPPETLNL
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.