||Recombinant humanCD274 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography.
||CD274, also known asprogrammed cell death 1 ligand 1(PDCD1L1), is involved in the costimulatorysignal, essential for T-cell proliferation and production of IL10 and IFNG,in an IL2-dependent and a PDCD1-independent manner. This protein interactionwith PDCD1 inhibits T-cell proliferation and cytokine production.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT
||27.9 kDa (245aa),confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
||> 90% by SDS -PAGE
||1 mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMFTVTV PKDLYVVEYG SNMTIECKFP VEKQLDLAAL IVYWEMEDKN IIQFVHGEEDLKVQHSSYRQ RARLLKDQLS LGNAALQITD VKLQDAGVYR CMISYGGADY KRITVKVNAP YNKINQRILVVDPVTSEHEL TCQAEGYPKA EVIWTSSDHQ VLSGKTTTTN SKREEKLFNV TSTLRINTTT NEIFYCTFRRLDPEENHTAE LVIPELPLAH PPNER
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.