Recombinant E.coli MsrA, His-tagged

• Cat.No.: msrA-988E
• Product Overview: Recombinant E.coli msrA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-174 aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: Peptide methionine sulfoxide reductase A, also known msrA, is an enzyme that catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. This protein could have an important function as a repair enzyme for proteins that have been inactivated by oxidation.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
• Molecular Mass: 25.4 kDa (232aa), confirmed by MALDI-TOF
• Purity: > 90 % by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay )
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: msrA methionine sulfoxide reductase A [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: msrA; ECK4215; JW4178; pms; pmsR; EG11433; MSRA; methionine sulfoxide reductase A; peptide met (O) reductase; cytosolic methionine-S-sulfoxide reductase; EC 1.8.4.11; PMSR; Peptide Met(O) reductase; Peptide-methionine (S)-S-oxide reductase; Protein-methionine-S-oxide reductase; methionine sulfoxide reductase A; peptide met (O) reductase
• Gene ID: 948734
• Protein Refseq: NP_418640
• UniProt ID: P0A744
• Function: N-terminus verified by Edman degradation on mature peptide; MsrA and MsrB are required to repair Ffh oxidized by reactive oxygen species produced by aerobic metabolism, establishing an as-yet undescribed link between protein targeting and oxidation