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Recombinant E.coli MsrA, His-tagged

Cat. No.: msrA-988E
Product Overview: Recombinant E.coli msrA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-174 aa).
Description: Peptide methionine sulfoxide reductase A, also known msrA, is an enzyme that catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. This protein could have an important function as a repair enzyme for proteins that have been inactivated by oxidation.
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Molecular Mass: 25.4 kDa (232aa), confirmed by MALDI-TOF
Purity: > 90 % by SDS-PAGE
Concentration: 0.5 mg/ml (determined by Bradford assay )
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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