||Recombinant humanTHAP1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
||THAPdomain-containing protein 1, also known as THAP1, is a 213 amino acid proteinthat localizes to the nucleoplasm and contains one THAPtype zinc finger, aconserved DNA-binding domain. It is a DNA-binding transcription regulatorthat regulates endothelial cell proliferation and G1/S cell-cycleprogression. THAP1 may also have pro-apoptopic activity by potentiating bothserum-withdrawal and TNF-induced apoptosis.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2M Urea, 10% glycerol, 1mM DTT, 0.2M NaCl.
||> 85%by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMVQSCS AYGCKNRYDK DKPVSFHKFP LTRPSLCKEW EAAVRRKNFKPTKYSSICSE HFTPDCFKRE CNNKLLKENA VPTIFLCTEP HDKKEDLLEP QEQLPPPPLP PPVSQVDAAIGLLMPPLQTP VNLSVFCDHN YTVEDTMHQR KRIHQLEQQV EKLRKKLKTA QQRCRRQERQ LEKLKEVVHFQKEKDDVSER GYVILPNDYF EIVEVPA
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.