Cat. No.: |
MAP2K2-1316H |
Product Overview: |
Recombinant humanMAP2K2 proetin, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques. |
Description: |
MAP2K2, also knownas MEK2,belongs to the STE grouping of kinases. Both MEK2 and the related MEK1 aredualspecificity kinases, phosphorylating and activating the mitogen-activatedprotein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptorsequence T-E-Y. MAP2K2 is activated by a wide variety of growth factors andcytokines and also by membrane depolarization and calcium influx. |
Source: |
E. coli |
Species: |
Human |
Form: |
Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol. |
Molecular Weight: |
46.9 kDa (424aa), confirmedby MALDI-TOF. |
Purity: |
> 90% by SDS -PAGE. |
Concentration: |
0.25 mg/ml(determined by Bradford assay). |
Sequences of aminoacids: |
MGSSHHHHHHSSGLVPRGSH MGSHMLARRK PVLPALTINP TIAEGPSPTS EGASEANLVD LQKKLEELEL DEQQKKRLEAFLTQKAKVGE LKDDDFERIS ELGAGNGGVV TKVQHRPSGL IMARKLIHLE IKPAIRNQII RELQVLHECNSPYIVGFYGA FYSDGEISIC MEHMDGGSLD QVLKEAKRIP EEILGKVSIA VLRGLAYLRE KHQIMHRDVKPSNILVNSRG EIKLCDFGVS GQLIDSMANS FVGTRSYMAP ERLQGTHYSV QSDIWSMGLS LVELAVGRYPIPPPDAKELE AIFGRPVVDG EEGEPHSISP RPRPPGRPVS GHGMDSRPAM AIFELLDYIV NEPPPKLPNG VFTPDFQEFVNKCLIKNPAE RADLKMLTNH TFIKRSEVEE VDFAGWLCKT LRLNQPGTPT RTAV |
Storage: |
Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles. |
OfficialSymbol: |
MAP2K2 |