Recombinant Human General Transcription Factor IIF, Polypeptide 2, 30kDa, His-tagged

• Cat.No.: GTF2F2-5599H
• Product Overview: Recombinant humanGTF2F2 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: GTF2F2, also knownas TFIIF, is a general transcription initiation factor that binds to RNApolymerase II and helps to recruit it to the initiation complex incollaboration with TFIIB. It promotes transcription elongation. This subunitshows ATP-dependent DNA-helicase activity.
• Form: Liquid. 20mMTris-HCl buffer (pH 8.0) containing 20% glycerol 0.2M NaCl, 1mM DTT.
• Molecular Weight: 30.5 kDa(269aa), confirmedby MALDI-TOF (Molecular weight on SDS-PAGE will appear higher).
• Purity: > 90% by SDS -PAGE.
• Concentration: 0.5mg/ml (determinedby Bradford assay).
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG RTEVSFTLNE DLANIHDIGGKPASVSAPRE HPFVLQSVGG QTLTVFTESS SDKLSLEGIV VQRAECRPAA SENYMRLKRL QIEESSKPVRLSQQLDKVVT TNYKPVANHQ YNIEYERKKK EDGKRARADK QHVLDMLFSA FEKHQYYNLK DLVDITKQPVVYLKEILKEI GVQNVKGIHK NTWELKPEYR HYQGEEKSD
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: GTF2F2

Gene Information

• Gene Name: GTF2F2 general transcriptionfactor IIF, polypeptide 2, 30kDa [ Homo sapiens ]
• Synonyms: GTF2F2; generaltranscription factor IIF, polypeptide 2, 30kDa; BTF4; RAP30; TF2F2;TFIIF; general transcription factor IIF subunit 2; TFIIF-beta; ATP-dependenthelicase GTF2F2; transcription initiation factor RAP30; general transcriptionfactor IIF 30 kDa subunit; transcription initiation factor IIF subunit beta
• Gene ID: 2963
• mRNA Refseq: NM_004128
• Protein Refseq: NP_004119
• MIM: 189969
• UniProt ID: P13984
• Chromosome Location: 13q14
• Pathway: Abortive elongationof HIV-1 transcript in the absence of Tat; Androgen Receptor SignalingPathway; Basal transcription factors; Eukaryotic Transcription Initiation;Formation and Maturation of mRNA Transcript; Formation of RNA Pol IIelongation complex; Gene Expression; Influenza Infection; Pausing andrecovery of HIV-1 elongation; RNA Pol II CTD phosphorylation and interactionwith CE; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening;Tat-mediated HIV-1 elongation arrest and recovery; Transcription; Viral MessengerRNA Synthesis; mRNA Splicing
• Function: ATP binding;ATP-dependent helicase activity; DNA binding; helicase activity; hydrolaseactivity; nucleotide binding; protein binding