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Active Recombinant Human EPHA4 protein(Met1-Thr547), His&hFc-tagged

Cat.No. : EPHA4-942H
Product Overview : Recombinant Human EPHA4 (NP_004429.1)(Met 1-Thr 547) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.
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Source : HEK293
Species : Human
Tag : C-His&hFc
Protein length : Met1-Thr547
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized human EPHA5 at 20 μg/ml (100 μl/well) can bind human EFNA4-Fc with a linear range of 1.28-32 ng/ml.
Molecular Mass : The recombinant human EPHA4/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 776 amino acids and predictes a molecular mass of 86.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EPHA4/Fc monomer is approximately 100-110 kDa due to high levels of glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 92 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : EPHA4 EPH receptor A4 [ Homo sapiens ]
Official Symbol : EPHA4
Synonyms : EPHA4; EPH receptor A4; EphA4 , TYRO1; ephrin type-A receptor 4; Hek8; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8; SEK; HEK8; TYRO1;
Gene ID : 2043
mRNA Refseq : NM_004438
Protein Refseq : NP_004429
MIM : 602188
UniProt ID : P54764

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does EPHA4 contribute to physiological processes and what is its specific role? 09/21/2021

EPHA4 is a member of the ephrin receptor family of protein kinases that plays an important role in neural development and synaptic plasticity. It is involved in axon guidance, neuronal migration, and cell proliferation.

What roles does the protein EPHA4 play in neuronal development? 01/19/2020

EPHA4 plays a critical role in neuronal development as a member of the Eph receptor family, which are receptor tyrosine kinases involved in various cellular processes. Specifically, EPHA4 regulates axon growth and guidance, acting as a repulsive cue to guide axons in the developing nervous system. Its involvement extends to synaptic plasticity and neuronal survival. Dysregulation of EPHA4 has been linked to neurological disorders, including Alzheimer's disease and neuropathic pain. Further research on the precise mechanisms and downstream signaling pathways of EPHA4 will deepen our understanding of its role in neuronal development and potential therapeutic applications.

How is EPHA4 expression regulated during development? 07/20/2019

EPHA4 expression is tightly regulated during nervous system development, with its expression gradually increasing during embryonic development. Additionally, EPHA4 expression is spatially regulated, with high levels in specific regions of the nervous system. Regulation of EPHA4 expression is mediated by several mechanisms, including transcriptional and post-transcriptional mechanisms.

How is EPHA4 involved in synaptic plasticity? 04/03/2018

EPHA4 is essential for normal synaptic plasticity, with its activation leading to the modulation of various synaptic proteins, such as AMPA receptors, NMDA receptors, and EphB receptors. Dysregulation of EPHA4-mediated signaling has been implicated in multiple neurodegenerative disorders, affecting both synaptic plasticity and axon growth.

What are the signaling mechanisms activated by EPHA4? 03/31/2018

EPHA4 signaling is initiated by binding to its ligands, which leads to activation of downstream signaling pathways. Several signaling pathways are activated by EPHA4, including the MAP kinase pathway, the PI3K-Akt pathway, and the RhoA-ROCK pathway, which play critical roles in the regulation of actin cytoskeleton dynamics.

Can EPHA4 be targeted therapeutically to treat neurodegenerative diseases? 04/07/2017

EPHA4 has emerged as a promising therapeutic target for various neurodegenerative diseases, and several pharmacological inhibitors have been developed to target EPHA4 signaling. These inhibitors have shown efficacy in preclinical models of several neurodegenerative diseases, suggesting their potential in clinical applications.

What is the role of genetic variants in the EPHA4 gene in neurodegenerative diseases? 09/29/2016

There is growing evidence that genetic variants in the EPHA4 gene may contribute to the susceptibility to neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. These variants have been shown to modify EPHA4 expression and function, and may contribute to disease pathogenesis via several mechanisms, including synaptic dysfunction and axon degeneration.

Customer Reviews (3)

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Reviews
03/11/2020

    The results interpretation process with this protein reagent is straightforward, making it suitable for beginners as well.

    10/25/2019

      The composition ratio of the reagent is highly accurate, ensuring the reliability of the experimental results.

      06/10/2016

        Astounding in both reliability and steadfastness, it confers irrefutable persuasion to the experiment.

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