||Recombinant mouse S100a6protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||S100calcium binding protein A6 (S100a6) belongsto the S100 family. S100 family is containing 2 EF-hand calcium-binding motifs.S100 proteins regulate a number of cellular processes such as cell cycle progressionsand differentiation. S100a6 plays a role inthe reorganization of the actin cytoskeleton and in cell mobility. This is a specifictarget of S-100B protein in vivo. The binding of S100a6 to S100B is stabilized by S100B-bound calcium and zinc.It associates with both Annexin XI and CacyBP (Calcyclin-binding protein). Itfunctions to activate several processes along the calcium signal transductionpathway including the regulation of cell growth, proliferation, secretion andexocytosis.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT ,30% glycerol, 100mM NaCl.
||12.2 kDa(109 aa), confirmed by MALDI-TOF.
||> 95%by SDS - PAGE
||0.5 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MACPLDQAIG LLVAIFHKYS GKEGDKHTLS KKELKELIQKELTIGSKLQD AEIARLMDDL DRNKDQEVNF QEYVAFLGAL ALIYNEALK
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.