||Recombinant humanUBE2W protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||UBE2Waccepts ubiquitin from the E1 complex and catalyzes its covalent attachmentto other proteins. This protein in vitro catalyzes monoubiquitination and"Lys-11"-linked polyubiquitination. UBE2W is widely expressed, especially athighest levels in testis.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
||19.5 kDa(171 aa), confirmed by MALDI-TOF.
||> 90%by SDS - PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYEGEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCKEKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.