||Recombinant MouseCarboxypeptidase E (rmCPE), Met1-Phe476 fused with 6×his tag at C-terminuswas expressed in human HEK293 cells
||CarboxypeptidaseE (CPE), also known as Carboxypeptidase H or enkephalin convertase.Carboxypeptidase E cleaves C-terminal amino acid residues and is involved inneuropeptide processing. It is a peripheral membrane protein. CPEspecifically binds regulated secretory pathway proteins, includingprohormones, but not constitutively secreted proteins. Carboxypeptidase Eappears to have several functions. The active form of carboxypeptidase E wasshown to be in secretory vesicles, where it acts as an exopeptidase toactivate neuropeptides, It does that by cleaving off basic C-terminal aminoacids, producing the active form of the peptide. Products of carboxypeptidaseE include insulin, enkephalin, and most other neuroendocrine peptides.It hasalso been proposed that membrane-associated carboxypeptidase E acts as asorting signal for regulated secretory proteins in the trans-Golgi network ofthe pituitary and in secretory granules; regulated secretory proteins aremostly hormones and neuropeptides. Mice with mutant carboxypeptidase E Cpe,display endocrine disorders like obesity and infertility.
||Human HEK293 cells
||Bulk protein in a0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulationor lyophilized powder.Normally 5-8% trehalose and mannitol are added asprotectants before lyophilization.
||>95% asdetermined by SDS-PAGE. All lots are greater than 95% pure.
||Store at -20℃ in lyophilizedstate after receipt. For long term storage, upon reconstitution rmCPE shouldbe aliquot and store at -20℃ or -80℃. Avoid repeated freeze-thaw cycles.