||Theprotein was expressed as 6*his-tagged fusion protein by E.Coli and puried byNi-sepharose. The puried protein was resolved in 1M P BS (58mM Na2HP O4,17mMNaH2P O4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl,15%glycerol
||Zinc finger proteinZPR1 is a protein that in humans is encoded by the ZNF259 gene
||58mM Na2HPO4,17mMNaH2PO4, 68mM NaCl, pH8
||1M PBS(58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and0.7% Sarcosyl, 15%glycerol.
||Store inPBS buffer at -20 oC. Repeated freeze thaw cycles should beavoided