||Recombinant Human AKT3 was expressed in E. coli. cells containing 6His tag at N-terminus and is purified by Ni-NTA agarose, 66kDa (47aa).
||The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
|amino acid sequence:
||15µg of recombinant protein in 100µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1% (v/v) 2-mercaptoethanol, 0.1mM EGTA, 1mM benzamidine, and 20% glycerol. Frozen solution.
||Stable for 6 months at -20°C.from date of shipment. For maximum yield, centrifuge the vial after thawing and prior to removing the cap.