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Active Recombinant Human TLR4 Protein, His-tagged

Cat.No. : TLR4-290H
Product Overview : Recombinant Human TLR4 with His tag was expressed in NS0 cell.
  • Specification
  • Gene Information
  • Related Products
Description : The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.
Source : Mammalian cells
Species : Human
Tag : His
Bio-activity : Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) TLR4 is Immobilized at 2 μg/mL (100 μL/well), the concentration of rhMD2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 μg/mL.
Molecular Mass : Predicted Molecular Weight: 70.6 kDa
SDS-PAGE: 90-95 kDa, reducing conditions
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method
Purity : >95% by SDS-PAGE
Storage : Store at -20 to -80 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reconstitution : Reconstitute at 100 μg/mL in PBS.
Gene Name : TLR4 toll like receptor 4 [ Homo sapiens (human) ]
Official Symbol : TLR4
Synonyms : TLR4; toll like receptor 4; TOLL; CD284; TLR-4; ARMD10; toll-like receptor 4; hToll; homolog of Drosophila toll; toll like receptor 4 protein; EC 3.2.2.6
Gene ID : 7099
mRNA Refseq : NM_003266
Protein Refseq : NP_003257
MIM : 603030
UniProt ID : O00206

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does TLR4 signaling intersect with the nuclear factor-kappa B (NF-κB) pathway? 11/20/2021

TLR4 signaling activates NF-κB, a transcription factor, promoting the expression of pro-inflammatory genes involved in immune responses.

Can you explain the molecular events following TLR4 activation upon ligand binding? 08/14/2020

Ligand binding induces conformational changes in TLR4, leading to the recruitment of adaptor proteins, such as MyD88, and the activation of downstream signaling cascades.

What is the role of the TLR4 signaling pathway in innate immunity? 02/18/2019

The TLR4 signaling pathway activates innate immune responses by detecting PAMPs, triggering the release of cytokines, and promoting the elimination of pathogens.

Can you elaborate on the crosstalk between TLR4 and other pattern recognition receptors (PRRs)? 10/02/2018

TLR4 engages in crosstalk with other PRRs, forming a network that enhances the sensitivity and specificity of the immune response to diverse pathogens.

Can you describe the molecular structure of TLR4 and its functional domains? 04/23/2018

TLR4 consists of extracellular leucine-rich repeats (LRRs) for ligand binding, a transmembrane domain, and an intracellular Toll/interleukin-1 receptor (TIR) domain for signaling.

Customer Reviews (3)

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Reviews
12/29/2020

    Precision in every experiment—this product is a game-changer for us.

    04/07/2019

      Timely delivery is key, and this product arrived on schedule.

      08/06/2018

        Exceptional customer service—knowledgeable staff guided us through the selection process.

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