Active Recombinant Mouse WNT3

• Cat.No.: WNT3-5160M
• Product Overview: Recombinant mouse WNT3 is a 38-41 kDa protein and has preferential expression in Cell culture.

Specification

• Cat. No.: WNT3-5160M
• Description: WNT3 belongs to the Wnt familyof signaling proteins that play a key role in maintaining the integrity ofembryonic and adult tissues. Expression of WNT3 occurs primarily alongthe dorsal midline across overlapping regions of the Central Nervous System(CNS). WNT3 signaling is essential for various morphogenetic events includingembryonic patterning, cell determination, cell proliferation, CNSdevelopment, and cytoskeletal formation.
• Source: Cell culture
• Species: Mouse
• Form: Supplied as alyophilized powder.
• Purity: > 75% pure by SDS PAGE and HPLC
• Sequence: Region of Wnt3 protein correspondingto amino acids SLAVGPQYSS LSTQPILCAS IPGLVPKQLR FCRNYVEIMP SVAEGVKAGIQECQHQFRGR RWNCTTVSNS LAIFGPVLDK ATRESAFVHA IASAGVAFAV TRSCAEGSAA ICGCSSRLQGSPGEGWKWGG CSEDIEFGGM VSREFADARE NRPDARSAMN RHNNEAGRQA IASHMHLKCK CHGLS
• Molecular Mass: 38-41 kDa
• Endotoxin Levels: Endotoxin level is less than 0.1 ngper ug (1 EU/ug).
• Bioactivity: The ED₅₀was determined by itsability to induced alkaline phosphatase production by CCL-226 cells. Theexpected ED50for this effect is 0.4-0.6 ng/ml.
• Applications: Numerous applications are possiblewith this product and should be tested by the end user under their ownlaboratory conditions.
• Storage: Store at 4°C until reconstitution. Following reconstitution aliquot and freeze at -20°C for long term storage. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: Wnt3
• Publication:
  WNT3 inhibits cerebellar granule neuron progenitor proliferation and medulloblastoma formation via MAPK activation. (2013)

Gene Information

• Gene Name: Wnt3 wingless-related MMTV integration site 3 [ Mus musculus ]
• Synonyms: WNT3; wingless-type MMTVintegration site family, member 3; INT4; MGC131950; MGC138321; MGC138323; proto-oncogene Wnt-3; WNT-3 proto-oncogeneprotein; proto-oncogene Int-4 homolog
• Gene ID: 22415
• mRNA Refseq: NM_009521
• Protein Refseq: NP_033547
• UniProt ID: P17553
• Chromosome Location: 11 E1
• Pathway: Basal cell carcinoma;Class B/2 (Secretin family receptors); DNA damage response (only ATMdependent); GPCR ligand binding; HTLV-I infection; Hedgehog signalingpathway; Melanogenesis; Pathways in cancer; Signaling by GPCR; Wnt SignalingPathway; Wnt Signaling Pathway NetPath; Wnt SignalingPathway and Pluripotency; Wnt signaling network
• Function: Frizzledbinding; frizzled-2 binding; receptor binding