||Recombinant human NXT1 protein, fused to His-tag at N-terminus, was expressed in E.coliand purified by using conventional chromatography (1-140aa).
||NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein) and CRM1 (required for chromosome region maintenance) dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. This protienheterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl
||18.0 kDa (160aa) confirmed by MALDI-TOF
||>85% by SDS - PAGE
||0.25 mg/ml (determined by BCAassay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MASVDFKTYV DQACRAAEEF VNVYYTTMDK RRRLLSRLYM GTATLVWNGN AVSGQESLSE FFEMLPSSEF QISVVDCQPV HDEATPSQTT VLVVICGSVK FEGNKQRDFN QNFILTAQAS PSNTVWKIAS DCFRFQDWAS
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.