||Recombinant human RhoB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-193aa).
||Rho-related GTP-binding protein RhoB, also known asRhoB,is a member of the Rho GTP-binding protein family. RhoB mediates apoptosis in neoplastically transformed cells after DNA damage. It plays a negative role in tumorigenesis as deletion causes tumor formation.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT,20% glycerol, 0.2mM NaCl
||23.9kDa (213aa), confirmed by MALDI-TOF(Molecular weight on SDS-PAGE will appear higher)
||> 90% by SDS - PAGE
||0.25mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.