Mouse Anti-Human Interleukin-33 Monoclonal Antibody
Cat.No. : | CAB11566MH |
Product Overview : | Mouse Monoclonal Antibody to Human Interleukin-33 |
- Specification
- Gene Information
- Related Products
Antigen Description : | Interleukin-33(IL-33), also known as DVS 27 and NF-HEV, is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyerss patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. IL-33 mediates its biological effects via Toll-interleukin 1 receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines. IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. |
Specificity : | Human IL33. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. |
Immunogen : | E.coli-derived rhIL-33 |
Host : | Mouse |
Isotype : | MouseIgG2a |
Species : | Human |
Cross Reactivity : | 5B4H5H9 |
Clone : | 6C5I6I10 |
Applications : | WB |
Dilution : | Western blot:This antibody can be used at 1 - 2μg /mL with the appropriate secondary reagents to detect IL-33 in WB. Using a DAB detection system, the detection limit for IL-33 is approximately 20ng/lane under reducing conditions and 100ng/lane non-reducing conditions. |
Preparation : | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant human IL-33 (rhIL-33; aa 112-270; NP_002296.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Format : | 0.2 μm filtered solution in PBS with 5% trehalose |
Storage : | This antibody can be stored at 2-8 °C for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Gene Name : | IL33 interleukin 33 [ Homo sapiens ] |
Official Symbol : | IL33 |
Synonyms : | IL33; interleukin 33; C9orf26,chromosome 9 open reading frame 26 (NF HEV); interleukin-33; DKFZp586H0523; DVS27; DVS27 related protein; IL1F11; interleukin 1 family; member 11; NF HEV; nuclear factor for high endothelial venules; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor from high endothelial venules; NF-HEV; NFEHEV; C9orf26; RP11-575C20.2; |
Gene ID : | 90865 |
mRNA Refseq : | NM_001199640 |
Protein Refseq : | NP_001186569 |
MIM : | 608678 |
UniProt ID : | O95760 |
Chromosome Location : | 9p24.1 |
Pathway : | Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Influenza A, organism-specific biosystem; Influenza A, conserved biosystem; |
Function : | cytokine activity; protein binding; |
Products Types
◆ Recombinant Protein | ||
IL33-5188H | Recombinant Human IL33 Protein, GST-tagged | +Inquiry |
Il33-157M | Recombinant Mouse Il33 Protein | +Inquiry |
IL33-356I | Active Recombinant Human IL33 Protein (159 aa) | +Inquiry |
IL33-2700R | Recombinant Rat IL33 Protein, His (Fc)-Avi-tagged | +Inquiry |
IL33-174H | Active Recombinant Human IL33 Protein | +Inquiry |
◆ Lysates | ||
IL33-5226HCL | Recombinant Human IL33 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (6)
Ask a questionIt can be used to detect immune cells such as macrophages and dendritic cells.
This protein applied in immunology, drug research, life science and other fields.
Regulates the activity of immune cells by binding to receptors to stimulate the production of inflammatory factors such as interferon and TNF-α.
IL33 can also be used to treat a variety of inflammatory diseases, such as inflammatory bowel disease, psoriasis and so on.
The preparation of IL33 requires first cloning the IL33 gene into the expression vector, and then the expression vector is expressed and purified in the cell culture medium.
Treating asthma with recombinant IL33 takes several weeks to several months to be effective, depending on the severity of the condition and the individual patient.
Customer Reviews (3)
Write a reviewGood solubility and is easy to operate and purify.
Effectively mimics the function of the target protein in vitro.
IL33 has a wide range of applications in the field of scientific research and is suitable for a variety of experimental design and research purposes.
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