| Antigen Description : |
Myelin oligodendrocyte glycoprotein (MOG) is a transmembrane protein belonging to immunoglobulin superfamily, and contains an Ig-like domain followed by two potential membrane-spanning regions. MOG is expressed only in the CNS with very low content (approximately 0.1% total proteins) in oligodendrogliocyte membrane. Three possible functions for MOG were suggested: (a) a cellular adhesive molecule, (b) a regulator of oligodendrocyte microtubule stability, and (c) a mediator of interactions between myelin and the immune system, in particular, the complement cascade. A direct interaction might exist between the membrane-associated regions of MOG and the myelin-specific glycolipid galactocerebroside (Gal-C), and such an interaction may have important consequences regarding the membrane topology and function of both molecules. It is considered that MOG is an autoantigen capable to produce a demyelinating multiple sclerosis-like disease in experimental animals. |
| Specificity : |
Human MOG. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. |
| Immunogen : |
Recombinant Human MOG Protein |
| Host : |
Rabbit |
| Isotype : |
Rabbit IgG |
| Species : |
Human |
| Clone : |
A014 |
| Applications : |
Western blot; ELISA |
| Dilution : |
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human MOG in WB.Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human MOG. The detection limit for Human MOG is approximately 0.00975 ng/well. |
| Preparation : |
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human MOG |
| Format : |
0.2 μm filtered solution in PBS with 5% trehalose |
| Storage : |
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
