Mouse Monoclonal Antibody to Human Plasminogen Activator, Urokinase
Cat.No. : | CAB11591MH |
Product Overview : | Mouse Monoclonal Antibody to Human Plasminogen Activator, Urokinase |
- Specification
- Gene Information
- Related Products
Antigen Description : | Plasminogen activator, urokinase, also known as PLAU and uPA, is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. The human PLAU is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain. PLAU is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. A specific polymorphism in PLAU gene is implicated in late-onset Alzheimer disease and also with decreased affinity for fibrin-binding. |
Specificity : | Human uPA / PLAU. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. |
Immunogen : | Recombinant human uPA protein |
Host : | Mouse |
Isotype : | MouseIgG1 |
Species : | Human |
Clone : | 2B3H5H12 |
Applications : | Western blot |
Dilution : | This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect A chain and full-length of uPA in WB. Using a DAB detection system, the detection limit for uPA is approximately 2ng/lane and 100ng/lane under non-reducing conditions and reducing conditions. |
Preparation : | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human uPA / PLAU. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Format : | 0.2 μm filtered solution in PBS with 5% trehalose |
Storage : | This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Gene Name : | PLAU plasminogen activator, urokinase [ Homo sapiens ] |
Official Symbol : | PLAU |
Synonyms : | PLAU; plasminogen activator, urokinase; urokinase-type plasminogen activator; UPA; URK; U-plasminogen activator; plasminogen activator, urinary; ATF; QPD; u-PA; BDPLT5; |
Gene ID : | 5328 |
mRNA Refseq : | NM_001145031 |
Protein Refseq : | NP_001138503 |
MIM : | 191840 |
UniProt ID : | P00749 |
Chromosome Location : | 10q24 |
Pathway : | ATF-2 transcription factor network, organism-specific biosystem; Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; DNA damage response (only ATM dependent), organism-specific biosystem; Dissolution of Fibrin Clot, organism-specific biosystem; |
Function : | peptidase activity; protein binding; serine-type endopeptidase activity; |
Products Types
◆ Recombinant Protein | ||
PLAU-6811M | Recombinant Mouse PLAU Protein, His (Fc)-Avi-tagged | +Inquiry |
PLAU-23H | Recombinant Human PLAU Protein (Ser21-Leu431), N-6×His tagged | +Inquiry |
PLAU-391C | Recombinant Cattle PLAU Protein, His-tagged | +Inquiry |
Plau-4908M | Recombinant Mouse Plau Protein, Myc/DDK-tagged | +Inquiry |
PLAU-806H | Recombinant Human PLAU Protein | +Inquiry |
◆ Native Protein | ||
PLAU-31687TH | Native Human PLAU | +Inquiry |
PLAU-31777TH | Native Human Human SERPINE1 | +Inquiry |
PLAU-31689TH | Active Native Human Urokinase protein | +Inquiry |
◆ Lysates | ||
PLAU-1734HCL | Recombinant Human PLAU cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0703 | PLAU (Human) Chromogenic Activity Assay Kit (Indirect Assay) | +Inquiry |
Kit-0702 | PLAU (Human) Chromogenic Activity Assay Kit (Direct Assay) | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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