Recombinant Human Growth Hormone 1 (Active) , His-tagged GH1-002N

Recombinant Human Growth Hormone 1 (Active) , His-tagged


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Recombinant Human Growth Hormone 1 (Active) , His-tagged

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Cat. No. : GH1-002N
Product Overview : It is produced by transient expression of GH in non-transgenic plant. The protein contains a 6His-tag in its N-terminal end. It is purified by sequential chromatography (IMAC and HIC). This product contains no animal –derived components or impurities.
Description : Growth Hormone (GH), is a protein produced by pituitary gland, is a member of the somatotropin / prolactin family of hormones which play an important role in growth control. This cytokine regulate growth and metabolism by binding to the GH receptor on the surface of many cell types. It act directly on the ends of the long bones promoting their growth and directly or indirectly act ,via IGF-1, on B cell, NK cells and macrophages to exert immunomodulatory activities. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization.
Source : Nicotiana benthamiana
Formulation : This product is lyophilized from a solution of Tris 0.05M, pH 7.4, NaCl 0.5M, and Triton X-100 0.1%.
Molecular formula : C1025H1570N280O306S7.
Extinction coefficient : E0.1% = 0,770 (A280 nm).
Molecular weight : 22.9 kDa
Purity assay : > 95% by SDS-PAGE gel.
Endotoxin level : < 0.01 EU/μg protein (LAL method)
Biological Activity : the biological activity is measured in a cell proliferation assay using the rat lynfoma, Nb2-11 (0.001-0.04 ng/mL).
Preparation instructions : It is recommended to reconstitute the lyophilized HGH in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Storage : Lyophilized GH, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, HGH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.
Gene Name : GH1 growth hormone 1 [ Homo sapiens ]
Official Symbol : GH1
Synonyms : GH1; growth hormone 1; GH; GHN; GH-N; hGH-N; IGHD1B; Pituitary growth hormone; growth hormone 1; GH1; IGHD1B; Somatotropin; hGH
Gene ID : 2688
mRNA Refseq : NM_000515
Protein Refseq : NP_000506
MIM : 604489
UniProt ID : P01241
Chromosome Location : 17q24.2
Function : growth factor activity; growth hormone activity; growth hormone receptor binding; prolactin receptor binding; protein binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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