Active Recombinant Human CTSC protein(Met1-Leu463), His-tagged

• Cat.No.: CTSC-2509H
• Product Overview: Recombinant Human CTSC (NP_001805.3) (Met 1-Leu 463) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Leu463
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Arg-7-amido-4-methylcoumarin (GRAMC). The specific activity is >200 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Cathepsin L for an activated form).
• Molecular Mass: The secreted recombinant human cathepsin C exists as the pro form after removal of the signal peptide. It consists of 450 amino acids and has a predicted molecular mass of 51 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCTSC is approximately 55 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CTSC cathepsin C [ Homo sapiens ]
• Official Symbol: CTSC
• Synonyms: CTSC; cathepsin C; PALS, PLS; dipeptidyl peptidase 1; DPP1; cathepsin J; dipeptidyl peptidase I; dipeptidyl transferase; dipeptidyl-peptidase I; JP; HMS; JPD; PLS; CPPI; DPPI; PALS; DPP-I
• Gene ID: 1075
• mRNA Refseq: NM_001114173
• Protein Refseq: NP_001107645
• MIM: 602365
• UniProt ID: P53634