Recombinant Mouse Dopa Decarboxylase, His-tagged

• Cat.No.: Ddc-2510M
• Product Overview: A DNA sequence encoding the mouse DDC (Met 1-Glu 480) was expressed,with a C-terminal polyhistidine tag. The recombinant mouse DDC consists of 490 amino acids and has a calculated molecular mass of 55.2 kDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions.

Specification

• Description: Ddc is a 54 kDa member of the group II decarboxylase family of proteins. It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3, 4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines.
• Source: E. coli
• Species: Mouse
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH8.0, 20%gly, 3mM DTT. Normally 5 %-8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Molecular Mass: 55.2 kDa
• Protein length: 490 aa
• Endotoxin: 1.0 eu/μg of the protein as determined by the lal
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 °C.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Ddc dopa decarboxylase [ Mus musculus ]
• Official Symbol: Ddc
• Synonyms: DDC; dopa decarboxylase; aromatic-L-amino-acid decarboxylase; Aadc
• Gene ID: 13195
• mRNA Refseq: NM_001190448
• Protein Refseq: NP_001177377
• Pathway: Amine-derived hormones, organism-specific biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Catecholamine biosynthesis, organism-specific biosystem; Catecholamine biosynthesis, tyrosine => dopamine =>
• Function: amino acid binding; aromatic-L-amino-acid decarboxylase activity; carboxy-lyase activity; catalytic activity; lyase activity; protein domain specific binding; pyridoxal phosphate binding