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Recombinant Mouse Glutathione S-Transferase, Mu 1, His-tagged

Cat.No.: Gstm1-2507M
Product Overview: Recombinant Gstm1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 28.1 kDa.
Description: Gstm1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.
Source: E. coli
Species: Mouse
Tag: His
Form: Liquid. In 20 mM Tris-HCl buffer ( pH8.0) containing 1mM DTT, 10% glycerol.
Bio-activity: Specific activity is < 11 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (cdnb) with reduced glutathione per minute at ph 6.5 at
Molecular Mass: 28.1 kDa (238aa), confirmed by MALDI-TOF
Protein length: 1-218aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK
Purity: > 95% by SDS-PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4? short term (1-2 weeks). For long term storage, aliquot and store at -20? or -70?. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)
Gene Name: Gstm1 glutathione S-transferase, mu 1 [ Mus musculus ]
Official Symbol: Gstm1
Synonyms: GSTM1; glutathione S-transferase, mu 1; glutathione S-transferase Mu 1; pmGT10; GST 1-1; GST class-mu 1; glutathione S-transferase GT8.7; glutathione-S-transferase, mu 1; Gstb1; Gstb-1;
Gene ID: 14862
mRNA Refseq: NM_010358
Protein Refseq: NP_034488
Pathway: Aflatoxin B1 metabolism, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; Estrogen metabolism, organism-specific biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem;
Function: glutathione transferase activity; protein binding; protein homodimerization activity; steroid binding; transferase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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