Recombinant Human Rho GDP Dissociation Inhibitor (GDI) Alpha, His-tagged

• Cat.No.: ARHGDIA-6906H
• Product Overview: Recombinant human ARHGDIA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 22.9 kDa.

Specification

• Description: ARHGDIA belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. Localized to the cytoplasm, ARHGDIA inhibits the dissociation of GDP from Rho proteins, thereby preventing GTP from binding to and subsequently activating Rho proteins. In humans ARHGDIA can be phosphorylated at Ser 101 by p21-activated kinase, an event that inhibits ARHGDIA activity and may result in positive feedback regulation of certain ARHGDIA target proteins.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
• Molecular Mass: 22.9 kDa (202aa) confirmed by MALDI-TOF
• Protein length: 24-204aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSVNYKPPAQ KSIQEIQELD KDDESLRKYK EALLGRVAVS ADPNVPNVVV TGLTLVCSSA PGPLELDLTG DLESFKKQSF VLKEGVEYRI KISFRVNREI VSGMKYIQHT YRKGVKIDKT DYMVGSYGPR AEEYEFLTPV EEAPKGMLAR GSYSIKSRFT DDDKTDHLSW EWNLTIKKDW KD
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ARHGDIA Rho GDP dissociation inhibitor (GDI) alpha [ Homo sapiens ]
• Official Symbol: ARHGDIA
• Synonyms: ARHGDIA; Rho GDP dissociation inhibitor (GDI) alpha; GDIA1; rho GDP-dissociation inhibitor 1; RHOGDI; rho GDI 1; rho-GDI alpha; RHOGDI-1; MGC117248
• Gene ID: 396
• mRNA Refseq: NM_001185077
• Protein Refseq: NP_001172006
• MIM: 601925
• UniProt ID: P52565
• Chromosome Location: 17q25.3
• Pathway: Axonal growth inhibition (RHOA activation), organism-specific biosystem; Axonal growth stimulation, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Neurotrophin signaling pathway, organism-specific biosystem; Neurotrophin signaling pathway, conserved biosystem; RAC1 signaling pathway, organism-specific biosystem; Regulation of CDC42 activity, organism-specific biosystem
• Function: GTPase activator activity; Rho GDP-dissociation inhibitor activity