Recombinant Human Proteasome (prosome, macropain) Subunit, Beta Type, 10, His-tagged

• Cat.No.: PSMB10-6864H
• Product Overview: Recombinant human PSMB10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 26.9 kDa.

Specification

• Description: PSMB10 is a member of the proteasome B-type family, also known as the T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl.
• Molecular Mass: 26.9 kDa (255aa), confirmed by MALDI-TOF.
• Protein length: 40-273aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MTTIAGLVFQ DGVILGADTR ATNDSVVADK SCEKIHFIAP KIYCCGAGVA ADAEMTTRMV ASKMELHALS TGREPRVATV TRILRQTLFR YQGHVGASLI VGGVDLTGPQ LYGVHPHGSY SRLPFTALGS GQDAALAVLE DRFQPNMTLE AAQGLLVEAV TAGILGDLGS GGNVDACVIT KTGAKLLRTL SSPTEPVKRS GRYHFVPGTT AVLTQTVKPL TLELVEETVQ AMEVE
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PSMB10 proteasome (prosome, macropain) subunit, beta type, 10 [ Homo sapiens ]
• Official Symbol: PSMB10
• Synonyms: PSMB10; proteasome (prosome, macropain) subunit, beta type, 10; MECL1; proteasome subunit beta type-10; beta2i; LMP10; MGC1665; proteasome MECl-1; macropain subunit MECl-1; proteasome subunit MECL1; proteasome subunit beta 7i; proteasome subunit beta-2i; low molecular mass protein 10; proteasome catalytic subunit 2i; multicatalytic endopeptidase complex subunit MECl-1; FLJ00366
• Gene ID: 5699
• mRNA Refseq: NM_002801
• Protein Refseq: NP_002792
• MIM: 176847
• UniProt ID: P40306
• Chromosome Location: 16q22.1
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem
• Function: peptidase activity; threonine-type endopeptidase activity