Recombinant Human MAD2 Mitotic Arrest Deficient-Like 1 (yeast), His-tagged

• Cat.No.: MAD2L1-6868H
• Product Overview: Recombinant human MAD2L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 25.7 kDa.

Specification

• Description: MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
• Molecular Mass: 25.7 kDa (225aa), confirmed by MALDI-TOF.
• Protein length: 1-205aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MALQLSREQG ITLRGSAEIV AEFFSFGINS ILYQRGIYPS ETFTRVQKYG LTLLVTTDLE LIKYLNNVVE QLKDWLYKCS VQKLVVVISN IESGEVLERW QFDIECDKTA KDDSAPREKS QKAIQDEIRS VIRQITATVT FLPLLEVSCS FDLLIYTDKD LVVPEKWEES GPQFITNSEE VRLRSFTTTI HKVNSMVAYK IPVND
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MAD2L1 MAD2 mitotic arrest deficient-like 1 (yeast) [ Homo sapiens ]
• Official Symbol: MAD2L1
• Synonyms: MAD2L1; MAD2 mitotic arrest deficient-like 1 (yeast); MAD2 (mitotic arrest deficient, yeast, homolog) like 1; mitotic spindle assembly checkpoint protein MAD2A; HSMAD2; MAD2; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
• Gene ID: 4085
• mRNA Refseq: NM_002358
• Protein Refseq: NP_002349
• MIM: 601467
• UniProt ID: Q13257
• Chromosome Location: 4q27
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Amplificationof signal from unattachedkinetochores via a MAD2inhibitory signal, organism-specific biosystem; Amplification of signal from the kinetochores, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem
• Function: protein binding; protein homodimerization activity