Recombinant Human Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1), His-tagged

• Cat.No.: CDKN1B-2499H
• Product Overview: Recombinant human CDKN1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 24.2 kDa.

Specification

• Description: CDKN1B, also known as cyclin-dependent kinase inhibitor 1B, belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. This protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. It is often referred to as a cell cycle inhibitor protein because its major function is to stop or slow down the cell division cycle.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol.
• Molecular Mass: 24.2 kDa (218aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• Protein length: 1-198aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSNVRVSNGS PS LERMDARQ AEHPKPSACR NLFGPVDHEE LTRDLEKHCR DMEEASQRKW NFDFQNHKPL EGKYEWQEVE KGSLPEFYYR PPRPPKGACK VPAQESQDVS GSRPAAPLIG APANSED THL VDPKTDPSDS QTGLAEQCAG IRKRPATDDS STQNKRANRT EENVSDGSPN AGSVEQTPKK PGLRRRQT
• Purity: >90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) [ Homo sapiens ]
• Official Symbol: CDKN1B
• Synonyms: CDKN1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; KIP1; P27KIP1; MEN4; CDKN4; MEN1B
• Gene ID: 1027
• mRNA Refseq: NM_004064
• Protein Refseq: NP_004055
• MIM: 600778
• UniProt ID: P46527
• Chromosome Location: 12p13.1-p12
• Pathway: AKT phosphorylates targets in the cytosol, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem
• Function: cyclin-dependent protein kinase activity; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase inhibitor activity; contributes_to cysteine-type endopeptidase activator activity involved in apoptotic process; kinase activity; protein binding; protein kinase inhibitor activity; protein phosphatase binding; transforming growth factor beta receptor, cytoplasmic mediator activity