"UBE2J2" Related Products

Recombinant Human Ubiquitin-Conjugating Enzyme E2, J2, His-tagged

Cat.No.: UBE2J2-2485H
Product Overview: Recombinant human UBE2J2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Description: UBE2J2 belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 30% glycerol, 1mM DTT, 200mM NaCl.
Molecular Mass: 28.0kDa (250aa) confirmed by MALDI-TOF
Protein length: 1-226aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSSTSS KRAPTTATQR LKQDYLRIKK DPVPYICAEP LPSNILEWHY VVRGPEMTPY EGGYYHGKLI FPREFPFKPP SIYMITPNGR FKCNTRLCLS ITDFHPDTWN PAWSVSTILT GLLSFMVEKG PTLGSIETSD FTKRQLAVQS LAFNLKDKVF CELFPEVVEE IKQKQKAQDE LSSRPQTLPL PDVVPDGETH LVQNGIQLLN GHAPGAVPNL AGLQQANRHH
Purity: >90% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration: 1mg/ml (determined by Bradford assay)
Gene Name: UBE2J2 ubiquitin-conjugating enzyme E2, J2 [ Homo sapiens ]
Official Symbol: UBE2J2
Synonyms: UBE2J2; ubiquitin-conjugating enzyme E2, J2; ubiquitin conjugating enzyme E2, J2 (UBC6 homolog, yeast); ubiquitin-conjugating enzyme E2 J2; NCUBE2; Ubc6p; yeast UBC6 homolog; ubiquitin conjugating enzyme 6; ubiquitin conjugating enzyme E2, J2; non-canonical ubiquitin-conjugating enzyme 2; ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast); NCUBE-2; PRO2121;
Gene ID: 118424
mRNA Refseq: NM_058167
Protein Refseq: NP_477515
UniProt ID: Q8N2K1
Chromosome Location: 1p36.33
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Parkinsons disease, organism-specific biosystem;
Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; ubiquitin-protein ligase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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